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Cytation 5 Cell Imaging Multi-Mode Reader

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Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows.;The multimode detection modules include filter- and monochromator-based fluorescence detection, luminescence, and UV-Vis absorbance detection. Gen5 software provides complete control over all imaging and data capture, plus powerful image and data analysis.


What's New

Application Note

Download our Application Note to discover how combining MitoXpress and pH-Xtra reagents with BioTek plate readers provide sensitive detection methods, including advanced lifetime (µs) time-resolved fluorescence (TRF), for getting the most meaningful data out of your cell metabolism assays.


Webinar OnDemand

A Powerful solution for resolving Seahorse XF Data using BioTek’s Cytation Cell Imaging Multi-Mode Readers


The Multi-Faceted Assays Enabled with Cytation Technology


Cell Culture & Imaging Microplates

Sourcing your microplates shouldn't be an added task in preparing your cell-based workflows. Agilent now offers cell culture and imaging microplates so you can complete your workflows seamlessly. Click here to learn more or buy now!


With the ability to collect phenotypic and quantitative data in a single experiment, Cytation 5 is a powerful tool for imaging applications, and conventional multi-mode applications.

Live Cell Proliferation and Viability Application Guide
Live Cell Proliferation and Viability


The AutoScratch Wound Making Tool automates cell migration and wound healing assays:

AutoScratch automatically makes consistent, repeatable scratch wounds

AutoScratch automatically makes consistent, repeatable scratch wounds.

The Scratch Assay App automatically calculates % confluence,  wound width and maximum healing rate (max V)

The Scratch Assay App automatically calculates % confluence, wound width and maximum healing rate (max V).

The Scratch Assay Starter kit enables automated scratch wound assays with excellent performance, compared to manual methods.

AutoScratch creates scratch wounds with excellent performance, compared to manual methods.

Rabbit spinal cord
Rabbit spinal cord with H&E dyes, stitched montage

SH Sy5y cells at 40x

These are just a few examples of the many important applications for Cytation 5. Visit our Applications page to see more.


BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!

Seahorse XF Imaging and Normalization System

Cytation 5 is a key component of Agilent Technologies’ Seahorse XF Imaging and Normalization System. XFe Analyzers simultaneously measure the two major cellular energy-producing pathways, mitochondrial respiration and glycolysis, in live cells and in real time. The integrated solution with Cytation 5 combines cellular analysis and imaging for an intuitive workflow to streamline XF data interpretation.

Automate Your Workflows

Expand your workflow automation by integrating the Cytation 5 with BioTek’s range of robotics, including the BenchCel Microplate Handler, BioStack™ Microplate Stacker and BioSpa™ Automated Incubator.


green box Multi-mode plate reader with sophisticated imaging

Multi-mode plate reader with sophisticated imaging

Cytation 5 extends the legacy of BioTek's multi-mode plate readers with a modular and upgradable imaging mode. Imaging opens up a range of applications for cell-based assays that cannot be performed on a standard plate reader. Information on cell morphology, localization of signal, cell count and more is obtained with Cytation 5’s imaging mode.

Plate reading: absorbance, fluorescence; luminescence; advanced read modes

Imaging: fluorescence; phase contrast; HC brightfield; brightfield; color brightfield

green box Cytation 5: Ready for any assay

Cytation 5: Ready for any assay

With its combination of hybrid plate reader and advanced microscopy mode, Cytation 5 is truly ready for any assay. Contact us to learn how Cytation 5 can transform your lab and greatly increase your productivity.

green box Advanced microscopy: Unlimited possibilities

Advanced microscopy: Unlimited possibilities

Cytation 5 automates many traditionally manual microscopy tasks, from slide scanning to time-lapse live cell assays; from low to high magnification. Cytation 5 is ready for any imaging assay.

Flexible hardware: 6-objective turret, 1.25x to 60x, 20+ colors available, wide FOV camera
Full automation: automated stage, autofocus, automated turret
Live cell imaging: temperature and gas (CO2 and O2) control for time-lapse live cell imaging

green box Hit-picking: Multi-mode detection + imaging saves time and data storage


(1) Plate reader quickly identifies GFP positive wells.
(2) Only GFP positive wells are imaged, saving both time and computer memory.

green box Powerful image processing and analysis

Powerful image processing and analysis

No need to process and analyze images one by one on a dedicated computer. In Gen5, pre-program your analysis tasks and walk away.

Image processing: stitching, Z-projection, deconvolution, digital phase contrast
Image analysis: cell count, confluence, cytoplasm analysis, intracellular analysis, subpopulation analysis, signal translocation and much more.


green box  Hybrid plate reader: Flexibility and performance

Hybrid plate reader: Flexibility and performance

With its patented combination of monochromator and filter optics, Cytation 5 is an advanced plate reader
that delivers both the flexibility and performance you need for any microplate assay in your lab.

Monochromator: variable bandwidth, absorbance, fluorescence, luminescence
Filters: fluorescence polarization, time-resolved fluorescence, Alpha laser

green box  Variable bandwidth for sensitivity and specificity

Variable bandwidth for sensitivity and specificity

The plate reader optics of Cytation uses a quad monochromator design with variable bandwidth. The bandwidth can be set anywhere between 9 and 50 nm in 1 nm increment. Large bandwidth settings (1) provide increased sensitivity and lower limits of detection. Small bandwidth settings (2) provide increased specificity when multiple signals are present, which reduces signal crosstalk and enhances assay performance.

green box Micro-volume analysis with Take3 plate

Micro-volume analysis with Take3 plate

Turn your Cytation 5 into a micro-volume analysis system with Take3. You can run 16 or 48 samples in one run to save a lot of time compared to single-sample devices. Gen5 is pre-programmed for ssDNA, dsDNA, RNA and protein quantification in 2 μL.

Product Reviews

Customer Spotlight

Institut de Pharmacologie Moléculaire et Cellulaire Activating Lasting Change in Childhood Cancer Treatments


In 2007, the Children’s Cancer Hospital Egypt (CCHE) 57357 was established to create a better tomorrow for children facing a devastating cancer diagnosis. As the largest pediatric oncology hospital in the world, the center offers best-in-class treatment therapies along with comprehensive family-centered quality care. In addition to clinical and wellness services, CCHE is also home to a wealth of cutting-edge researchers focused on improving clinical outcomes and reducing adverse therapeutic events.





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