VIAFLO 96/384 Handheld Electronic 24, 96 And 384 Channel Pipette
Handheld Electronic 24, 96 And 384 Channel Pipette
Increase your pipetting throughput and reproducibility without investing in big automation
Previously, high throughput microplate pipetting was only possible by investing in fully automated liquid handling robots. These robots are not only expensive, they are also complex and difficult to program, requiring specially trained users.
VIAFLO 96/384 is the solution. With this 24, 96 and 384 channel pipette, INTEGRA meets the need for an affordable, compact and easy to use pipette that provides increased productivity. The unique operating concept makes VIAFLO 96/384 as easy to use as any traditional handheld pipette.
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Show allHow it works
VIAFLO 96 and VIAFLO 384 enable transfers of 24, 96 and 384 samples in a single step, thus increasing pipetting throughput and reproducibility significantly. At a fraction of the cost, they often surpass throughput capabilities of even expensive robots.
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Intuitive handheld operation
![Pipetting system ensures rapid, controllable and reproducible handling of cell cultures]()
VIAFLO 96/384 is a handheld 24, 96 or 384 channel pipette designed to fit on any lab bench. It features a unique operating concept making it as easy to use as a traditional handheld pipette. Hold the control unit and push to move the pipetting head to the desired pipetting position. This unique servo assisted steering mechanism allows fast, precise and stress free multichannel pipetting without the need for position programming.
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Quick and comprehensible menu navigatIon
![Adjusting the volume of the VOYAGER electronic pipette is easy and intuitive with the use of the touch wheel.]()
All INTEGRA electronic pipettes have a common user interface making it easy to switch between different systems without a second thought.
A full color screen and proven touch wheel interface offer quick and comprehensible menu navigation. Simply by moving the finger over the touch wheel, menus and settings can be accessed and changed.VIAFLO 96 and VIAFLO 384 can be connected to a computer via USB port. With the free to use VIALINK PIPETTE MANAGEMENT SOFTWARE, user defined custom programs can be created on the computer and then be transferred onto VIAFLO 96/384.
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Always work in the optimal volume range
![Change pipetting head to change pipetting volume range]()
Users can simply change the pipetting head of their VIAFLO 96 and VIAFLO 384 to guarantee optimal matching of the available volume range to the application performed. Changing a pipetting head of VIAFLO 96 and VIAFLO 384 is easy and takes less than one minute.
Each channel of a pipetting head is independent, having its own precise piston and sealing to exclude any cross-channel influences for error-free pipetting.
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Space Saving Footprint
![Space-saving design allows use under an extractor hood]()
With its compact design, this benchtop system requires little space on a lab bench and fits into most laminar flow hoods to allow work under sterile conditions. Up to three stage positions accommodate tip racks, microplates, reagent reservoirs or other SBS size labware.
Up to 8 microplates can be stacked to make maximum use of the stage positions.
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GripTip Pipette Tips Which Never Leak or Fall off
![Award winning benchtop pipetting system]()
Losing a tip during an assay preparation can ruin hours of work. Also a reliable sealing between tip and fitting to avoid dripping tips is of key importance for accurate pipetting.
INTEGRA has redesigned the connection between pipette tip and tip fitting to eliminate these issues. The unique GRIPTIP PIPETTE TIPS are comfortably loaded and ejected without any physical effort due to a power assisted mechanism activated by a push button. The loading procedure also guarantees that GripTips are not touched by the user and remain sterile.
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Uses and Applications
Applications
- Reservoir to plate transfers
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Reservoir to plate transfers
VIAFLO 96/384 fills microplates within seconds by transferring up to 384 samples simultaneously. It reduces pipetting errors and increases pipetting reproducibility as all samples are transferred the same way.
![Time to fill five 96 well microplates with VIAFLO 96 compared to single and 8-channel pipette]()
Time to fill five 96 well microplates [seconds] The “Repeat dispense” function is an extremely effective mode to fill multiple plates with the same liquid: Aspirate once and dispense multiple aliquots of the same or different volume. Plates can be stacked during the process to minimize plate handling.
Typical applications of reservoir to plate transfers are:
- Compound additions
- Medium exchange
- Cell seeding
- Addition of buffers and reagents (e.g. ELISA handling)
![Repeat dispense with VIAFLO 96 channel pipette]()
Repeat dispense with VIAFLO 96 - Plate to plate transfers
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Plate to plate transfers
Plate duplication, supernatant transfers and plate reformatting
- Plate duplication
- Supernatant transfers
- Plate reformatting 24 to 96 well plate
- Plate reformatting 96 to 384 well plate
- Plate reformatting 384 to 1536 well plate
Find the right plate holder option
An exact aspiration height can be defined to precisely remove supernatant. The ability to adjust the pipetting speed helps to gently aspirate without disturbing cells at the bottom of a well.
![The VIAFLO 96/384 is handheld, electronic pipette]()
Plate to plate transfer using a VIAFLO 384 - Transfer within plates
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Transfer within plates
Performing serial dilutions, mixing or resuspending contents
Performing serial dilutions, mixing or resuspending contents of entire plates can be effortlessly accomplished without repeatedly pressing a plunger. The number of mixing cycles and the mixing speed can be exactly defined.
![Serial dilution using a VIAFLO 96 channel pipette]()
Serial dilutions using a VIAFLO 96 - Nucleotide and protein extraction
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Nucleotide and protein extraction
Video demonstration
- 24 well pipetting
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24 well pipetting
Video explains how 24 well plates can be quickly, easily, and efficiently filled using the 24 channel pipetting head
- 1536 well pipetting
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1536 well pipetting
Video explains how the special 1536 well plate holder enables easy and fast 1536 well pipetting with VIAFLO 384
- High throughput screening
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High throughput screening
Molecular Partners from Zurich (CH) integrated a VIAFLO 96 in their protein expression workflow. The pipette is used for various steps in their IMAC protein purification protocol.
Integrating an INTEGRA VIAFLO 96 multichannel pipette into their high throughput expression facility has enabled Molecular Partners (Zurich, Switzerland) to streamline the discovery and development of a novel class of targeted protein therapeutics termed DARPins.
DARPins are a next generation of protein therapeutics combining the advantages of antibodies and small molecule drugs. High target specificity and potency, small size, cost-effective production and a whole range of possible multi-specific format choices gives them the potential to surpass existing antibody drugs and revolutionize protein therapeutics.
Andreas Lehmann, an Expert Technical Assistant working in Molecular Partners 96 well high throughput expression facility commented:
“Our lab is extremely satisfied with our decision to purchase and incorporate INTEGRA’s VIAFLO 96 into our development protocols. The main application we are using the system for currently is protein purification using IMAC (Immobilized Metal Affinity Chromatography). The VIAFLO 96 is used for various steps in the process starting from the preparation of bacterial cultures right through to the actual purification. Overall our protocol involves 63 full plate liquid transfers; such a workload would not be feasible with a traditional handheld multichannel pipette. We have found that the VIAFLO 96 is easy to integrate into our standard operating protocols because parameters can be defined: pipetting mode, volume, pipetting speed and pipetting height. We particularly like the repeat dispense function on the VIAFLO 96 as it saves the lab a lot of time and improves the overall reproducibility of tests as all samples processed at the same time in the same way".
Mr. Lehmann added:
"Being responsible for training of new staff, I also appreciate that the VIAFLO 96 is user friendly and intuitive to use because very little training is required".
- Efficient Library Screening
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Efficient Library Screening
PepsiCo Global R&D from White Plains NY (USA) uses a VIAFLO 384 to screen natural product libraries to find sugar substitutes. The 384 channel pipette is used to transfer compound plates to multiple cell plates.
- Improving Efficiency of Replication & Screening
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Improving Efficiency of Replication & Screening
Using a VIAFLO 96 handheld multichannel pipette, the Institute of Genetics and Development of Rennes (IGDR) in France has been able to improve the efficiency with which they replicate yeast strain collections and perform genetic screens in 96 well plates.
The Institute of Genetics and Development of Rennes (www.igdr.univ-rennes1.fr) is one of the major biological research institutes in France. The objective of the IGDR is to develop research of excellence in areas including Genetics, Cell Biology and Developmental Biology. Employing 20 teams of scientists – current research interests at IGDR include investigations into cell polarity, cell cycle, signalisation, membrane trafficking, RNA stability, splicing, epigenetics, genetics and transcription. IGDR also study human pathologies like cancers, and genetics and orphan diseases.
Ewa Blaszczak, a PhD researcher at IGDR commented:
“We purchased the liquid handler from INTEGRA Biosciences nearly three years ago and are very pleased with it. We use it to replicate collections and yeast strains and to perform genetic screens in 96 well plates. Thanks to the speed and efficiency of the VIAFLO 96 we can, for instance, replicate a whole collection of 5784 yeast strains in less than one week".
She added:
"Our VIAFLO 96 has proven to be a great liquid handling device not only because of the speed and efficiency but also for its practicality. Set up is simple and operation intuitive. You can set-up a repeat mixing and dispensing application as well as change the aspirated liquid volume at any time. Moreover, its compact size allows you to fit it easily into any almost any laboratory space. I would strongly recommend the VIAFLO 96 for any type of 96 or 384 well plate applications”.
- Cell culture workflow
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Cell culture workflow
Streamlining and standardizing cell culture workflows using the INTEGRA VIAFLO 96/384
- Heating, Cooling & Shaking
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Heating, Cooling & Shaking
Options to allow incubation and shaking at different temperatures.
Active Heating & Cooling
Heating and Cooling blocks which can be easily integrated into the VIAFLO 96/384 in order to help you perfectly control your application's temperatures.
For more information about this product please contact your local sales representative.
Passive cooling
96 and 384 well PCR aluminum cooling blocks to keep reagents in various PCR plates chilled. In addition, the 96-well cooling block can also accommodate 0.2 ml PCR tubes.
![96- and 384-well PCR cooling blocks.]()
PCR Cooling Blocks Shaking
Shaking devices can be accomodated on the VIAFLO 96/384, if your application also includes shaking steps. Combined heating and shaking options are available.
![VIAFLO 96/384 Heating & Shaking solution for diffrent labware types]()
Heating & Shaking on the VIALFO 96/384
Tech info
Find here a quick overview of the product specifications. More detailed information can be found in the download-section below.
- Dimensions (W x D x H)
- 42 cm x 30 cm x 54 cm
- Power Requirements
- 100–240 VAC, 50/60 Hz
- Weight of VIAFLO 96 incl. pipetting head
- 25.7 kg
- Weight of VIAFLO 384 incl. pipetting head
- 27.7 kg
- Pipetting channels of VIAFLO 96
- 96, 24
- Pipetting channels of VIAFLO 384
- 384, 96, 24
- Compatible plate formats
- 24, 96, 384, 1536 wells, shallow and deep well
- Plate positions
- up to 3
Parts and numbers
- Instruments
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Instruments
VIAFLO 96 and VIAFLO 384 Handheld Pipette
Description Part No. VIAFLO 96, 24 and 96 channel handheld pipette (base unit only) 6001 VIAFLO 384, 24, 96 and 384 channel handheld pipette (base unit only) 6031 ![VIAFLO 96/384 channel pipette base units]()
VIAFLO 96/384 base units - Plate Holders
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Plate Holders
Plate Holders for 24, 96, 384 and 1536 well plate
Find the right plate holder option
Description Part No. Standard plate holder for 24 and 96 well plate 6205 Plate holder for 24, 96 and 384 well plates, with slide function (384 offset) 6210 Spring loaded plate holder A for 24, 96 and 384 well plates, with slide function (384 offset) 6215 Spring loaded plate holder B for 24, 96 and 384 well plates, with slide function (384 offset) 6220 Row dilution plate holder for 24, 96 and 384 well plates 6221 Plate holder for 24 channel head 6222 Adjustable spring loaded plate holder A for 24, 96 and 384 well plates, with slide function (384 offset) 6223 Adjustable spring loaded plate holder B for 24, 96 and 384 well plates, with slide function (384 offset) 6224 Adjustable spring loaded plate holder A for 24, 96, 384 and 1536 well plates, with slide function (1536 offset) 6225 Adjustable spring loaded plate holder B for 24, 96, 384 and 1536 well plates, with slide function (1536 offset) 6226 Three position stage for 96 and 384 well plates (occupies both deck positions A and B) 6230 ![Plate Holders for 96-, 384- and 1536-well plates]()
Plate Holders for 24, 96, 384 and 1536 well plates - Pipetting Heads
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Pipetting Heads
24, 96 and 384 Channel Pipetting Heads
24 Channel Pipetting Heads (for VIAFLO 96 and VIAFLO 384)
Volume Range Part No. 10 – 300 µl 6123 50 – 1250 µl 6124 ![Tête de pipetage 24 canaux pour VIAFLO 96/384]()
24 channel pipetting head 96 Channel Pipetting Heads (for VIAFLO 96 and VIAFLO 384)
Volume Range Part No. 0.5 – 12.5 µl 6101 2 – 50 µl 6106 5 – 125 µl 6102 10 – 300 µl 6103 50 – 1250 µl 6104 ![96 Channel Pipetting Heads]()
96 channel pipetting heads 384 Channel Pipetting Heads (only for VIAFLO 384)
Volume Range Part No. 0.5 – 12.5 µl 6131 2 – 50 µl 6136 5 – 125 µl 6132 ![VIAFLO 96/384 - 384 channel head]()
384 channel pipetting heads - Automation Friendly Clear Advantage™ Reservoirs
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Automation Friendly Clear Advantage™ Reservoirs
Reservoir bases, lids and disposable reagent reservoirs
INTEGRA offers two types of automation friendly reagent reservoirs:
- 150 ml and 300 ml Clear Advantage™ reagent reservoirs which are made of both crystal clear virgin polystyrene and polypropylene. Both options fit into a reusable automation friendly base with bold, crisp, clearly visible graduation markings.
- The 300 ml open well, 12 column and 8 row polypropylene reservoir blocks offer improved chemical compatibility.
![150 ml trial pack]()
150 ml Reagent Reservoir Trial Pack - 150 ml and 300 ml Clear Advantage™ reagent reservoirs which are made of both crystal clear virgin polystyrene and polypropylene. Both options fit into a reusable automation friendly base with bold, crisp, clearly visible graduation markings.
- Heating, Cooling, Shaking
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Heating, Cooling, Shaking
Options to allow incubation or shaking at different temperatures
Passive cooling
96 and 384 well PCR aluminum cooling blocks to keep reagents in various PCR plates chilled. In addition, the 96-well cooling block can also accommodate 0.2 ml PCR tubes.
Description Part No. 96 well PCR cooling plate 6250 384 well PCR cooling plate 6255 Active heating, cooling & shaking
Shaking devices can be accomodated on the VIAFLO 96/384, if your application also includes shaking steps. Combined heating and shaking options are available.
Description Part No. INHECO Heating & Cooling Package Contact your local sales representative INHECO Shaking Package Contact your local sales representative - Replacement O-Rings
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Replacement O-Rings
Red O-Rings for Tip Fittings
Description Part No. 200/300 µl, replacement kit, 24/pack 100-00027-50 200/300 µl, replacement kit, 100/pack 125928 1000/1250 µl, replacement kit, 24/pack 100-00028-50 1000/1250 µl, replacement kit, 100/pack 125929 - Cover
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Cover
Protection Cover for VIAFLO 96 & VIAFLO 384
Description Part No. Protection Cover for VIAFLO 96 & VIAFLO 384 6280 - GripTips
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GripTips
Use the GripTip Selector Guide to find your pipette tips.
