VIAFLO 96 | VIAFLO 384 24, 96 And 384 Channel Handheld Electronic Pipettes

Applications

Reservoir to plate transfers

Reservoir to plate transfers

VIAFLO 96 and VIAFLO 384 fill microplates within seconds by transferring up to 384 samples simultaneously. It reduces pipetting errors and increases pipetting reproducibility as all samples are transferred the same way.

The “Repeat dispense” function is an extremely effective mode to fill multiple plates with the same liquid: Aspirate once and dispense multiple aliquots of the same or different volume. Plates can be stacked during the process to minimize plate handling.

Typical applications of reservoir to plate transfers are:

• Compound additions
• Medium exchange
• Cell seeding
• Addition of buffers and reagents (e.g. ELISA handling)

Plate to plate transfers

Plate to plate transfers

Plate duplication, supernatant transfers and plate reformatting

• Plate duplication
• Supernatant transfers
• Plate reformatting 24 to 96 well plate
• Plate reformatting 96 to 384 well plate
• Plate reformatting 384 to 1536 well plate

Find the right plate holder option

An exact aspiration height can be defined to precisely remove supernatant. The ability to adjust the pipetting speed helps to gently aspirate without disturbing cells at the bottom of a well.

 

If you would like to learn more about plate to plate transfers with the VIAFLO 96 and VIAFLO 384 handheld electronic pipettes and other electronic pipettes of INTEGRA, see this application note: Plate to plate transfer

 

Transfer within plates

Transfer within plates

Performing serial dilutions, mixing or resuspending contents

Performing serial dilutions, mixing or resuspending contents of entire plates can be effortlessly accomplished without repeatedly pressing a plunger. The number of mixing cycles and the mixing speed can be exactly defined.

Nucleic acid extraction

Nucleic acid extraction

Video demonstration

Solid phase extraction with the VIAFLO 96 and VIAFLO 384 and the BIORAD vacuum manifold

24 well pipetting

24 well pipetting

Video explains how 24 well plates can be quickly, easily, and efficiently filled using the 24 channel pipetting head

VIAFLO 96 / VIAFLO 384 - 24 channel head

1536 well pipetting

1536 well pipetting

Video explains how the special 1536 well plate holder enables easy and fast 1536 well pipetting with VIAFLO 384

VIAFLO 384 - Plate Holder For 1536 well Plates

High throughput screening

High throughput screening

Molecular Partners from Zurich (CH) integrated a VIAFLO 96 in their protein expression workflow. The pipette is used for various steps in their IMAC protein purification protocol.

Integrating an INTEGRA VIAFLO 96 multichannel pipette into their high throughput expression facility has enabled Molecular Partners (Zurich, Switzerland) to streamline the discovery and development of a novel class of targeted protein therapeutics termed DARPins.

DARPins are a next generation of protein therapeutics combining the advantages of antibodies and small molecule drugs. High target specificity and potency, small size, cost-effective production and a whole range of possible multi-specific format choices gives them the potential to surpass existing antibody drugs and revolutionize protein therapeutics.

Andreas Lehmann, an Expert Technical Assistant working in Molecular Partners 96 well high throughput expression facility commented:

“Our lab is extremely satisfied with our decision to purchase and incorporate INTEGRA’s VIAFLO 96 into our development protocols. The main application we are using the system for currently is protein purification using IMAC (Immobilized Metal Affinity Chromatography). The VIAFLO 96 is used for various steps in the process starting from the preparation of bacterial cultures right through to the actual purification. Overall our protocol involves 63 full plate liquid transfers; such a workload would not be feasible with a traditional handheld multichannel pipette. We have found that the VIAFLO 96 is easy to integrate into our standard operating protocols because parameters can be defined: pipetting mode, volume, pipetting speed and pipetting height. We particularly like the repeat dispense function on the VIAFLO 96 as it saves the lab a lot of time and improves the overall reproducibility of tests as all samples processed at the same time in the same way".

Mr. Lehmann added:

"Being responsible for training of new staff, I also appreciate that the VIAFLO 96 is user friendly and intuitive to use because very little training is required".

Efficient Library Screening

Efficient Library Screening

PepsiCo Global R&D from White Plains NY (USA) uses a VIAFLO 384 to screen natural product libraries to find sugar substitutes. The 384 channel pipette is used to transfer compound plates to multiple cell plates.

PepsiCo Global R&D uses a VIAFLO 384 to screen natural product libraries

Improving Efficiency of Replication & Screening

Improving Efficiency of Replication & Screening

Using a VIAFLO 96 handheld multichannel pipette, the Institute of Genetics and Development of Rennes (IGDR) in France has been able to improve the efficiency with which they replicate yeast strain collections and perform genetic screens in 96 well plates.

The Institute of Genetics and Development of Rennes (www.igdr.univ-rennes1.fr) is one of the major biological research institutes in France. The objective of the IGDR is to develop research of excellence in areas including Genetics, Cell Biology and Developmental Biology. Employing 20 teams of scientists – current research interests at IGDR include investigations into cell polarity, cell cycle, signalisation, membrane trafficking, RNA stability, splicing, epigenetics, genetics and transcription. IGDR also study human pathologies like cancers, and genetics and orphan diseases.

Ewa Blaszczak, a PhD researcher at IGDR commented:

“We purchased the liquid handler from INTEGRA Biosciences nearly three years ago and are very pleased with it. We use it to replicate collections and yeast strains and to perform genetic screens in 96 well plates. Thanks to the speed and efficiency of the VIAFLO 96 we can, for instance, replicate a whole collection of 5784 yeast strains in less than one week".

She added:

"Our VIAFLO 96 has proven to be a great liquid handling device not only because of the speed and efficiency but also for its practicality. Set up is simple and operation intuitive. You can set-up a repeat mixing and dispensing application as well as change the aspirated liquid volume at any time. Moreover, its compact size allows you to fit it easily into any almost any laboratory space. I would strongly recommend the VIAFLO 96 for any type of 96 or 384 well plate applications”.

Cell culture workflow

Cell culture workflow

Streamlining and standardizing cell culture workflows using the INTEGRA VIAFLO 96 and VIAFLO 384

Cell culture workflow

Heating, Cooling & Shaking

Heating, Cooling & Shaking

Options to allow incubation and shaking at different temperatures.

Active Heating & Cooling

Heating and Cooling blocks which can be easily integrated into the VIAFLO 96 or VIAFLO 384 in order to help you perfectly control your application's temperatures.

For more information about this product please contact your local sales representative.

Heating & Cooling

Passive cooling

Our passive, iceless cooling blocks keep buffers, enzymes and samples uniformly chilled and ready for use, giving you more time for preparation and processing of your experiments before enzyme or DNA samples begin to degrade. The 96-well cooling block can also accommodate 0.2 ml PCR tubes. 

Shaking

Shaking devices can be accomodated on the VIAFLO 96 or VIAFLO 384, if your application also includes shaking steps. Combined heating and shaking options are available.