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Mastercycler® X50

Mastercycler<sup>&reg;</sup>&nbsp;X50 PCR thermocycler - Front view
Mastercycler<sup>&reg;</sup>&nbsp;X50 PCR thermocycler - Touch screen interface
Mastercycler<sup>&reg;</sup>&nbsp;X50 touchscreen and eco PCR thermocycler range, including X50h and X50r - Open units
Scientist using Mastercycler<sup>&reg;</sup> X50 PCR thermocyclers in the lab.
Scientist using Mastercycler<sup>&reg;</sup>&nbsp;X50 PCR thermocyclers in the lab
Mastercycler<sup>&reg;</sup>&nbsp;X50 units networked in the lab - Open units
Scientist programing Mastercycler<sup>&reg;</sup>&nbsp;X50 PCR thermocycler via touch screen interface in the lab
Mastercycler<sup>&reg;</sup>&nbsp;X50 PCR 2D heatmap - Left to Right
Mastercycler<sup>&reg;</sup>&nbsp;X50 2D heatmap - Top to Bottom
Mastercycler<sup>&reg;</sup>&nbsp;X50h PCR thermocycler - Open unit, top-down view
Scientist loading samples into Mastercycler<sup>&reg;</sup>&nbsp;X50 eco unit
Mastercycler<sup>&reg;</sup>&nbsp;X50 PCR thermocycler in lab - Closed unit, front view showing small footprint
Mastercycler<sup>&reg;</sup>&nbsp;X50h PCR thermocycler - Closed unit, top-down view
Mastercycler<sup>&reg;</sup>&nbsp;X50t eco PCR thermocycler unit - Front view
Mastercycler<sup>&reg;</sup>&nbsp;X50 PCR thermocycler in lab - Open unit, front view showing flexlid<sup>&reg;</sup>
Mastercycler<sup>&reg;</sup>&nbsp;X50 PCR thermocycler touch screen interface with active status display
Mastercycler<sup>&reg;</sup>&nbsp;X50 PCR thermocycler - Front view
Mastercycler<sup>&reg;</sup>&nbsp;X50 PCR thermocycler - Touch screen interface
Mastercycler<sup>&reg;</sup>&nbsp;X50 touchscreen and eco PCR thermocycler range, including X50h and X50r - Open units
Scientist using Mastercycler<sup>&reg;</sup> X50 PCR thermocyclers in the lab.
Scientist using Mastercycler<sup>&reg;</sup>&nbsp;X50 PCR thermocyclers in the lab
Mastercycler<sup>&reg;</sup>&nbsp;X50 units networked in the lab - Open units
Scientist programing Mastercycler<sup>&reg;</sup>&nbsp;X50 PCR thermocycler via touch screen interface in the lab
Mastercycler<sup>&reg;</sup>&nbsp;X50 PCR 2D heatmap - Left to Right
Mastercycler<sup>&reg;</sup>&nbsp;X50 2D heatmap - Top to Bottom
Mastercycler<sup>&reg;</sup>&nbsp;X50h PCR thermocycler - Open unit, top-down view
Scientist loading samples into Mastercycler<sup>&reg;</sup>&nbsp;X50 eco unit
Mastercycler<sup>&reg;</sup>&nbsp;X50 PCR thermocycler in lab - Closed unit, front view showing small footprint
Mastercycler<sup>&reg;</sup>&nbsp;X50h PCR thermocycler - Closed unit, top-down view
Mastercycler<sup>&reg;</sup>&nbsp;X50t eco PCR thermocycler unit - Front view
Mastercycler<sup>&reg;</sup>&nbsp;X50 PCR thermocycler in lab - Open unit, front view showing flexlid<sup>&reg;</sup>
Mastercycler<sup>&reg;</sup>&nbsp;X50 PCR thermocycler touch screen interface with active status display

The new Mastercycler® X50 elegantly combines speed, flexibility, and ease of use. With its intuitive touch screen offering precise fingertip control, the MC X50 PCR thermocycler enables easy PCR optimization in advanced molecular biology research and dependable standardization in routine PCR applications. It is also perfectly fitted for NGS. More information

Image – German Design Award Winner 2018
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Product Information

Available as standalone units, controlled by the user-friendly VisioNize® touchscreen interface, or externally controlled eco units, the Mastercycler X50 family offers the flexibility to meet all your throughput needs.

Each standalone PCR thermocycler can be networked with up to nine additional eco units. For even higher throughput, you can control up to fifty MC X50 eco units with our CycleManager X50 software. Whichever you need, you’ll be able to monitor and manage every unit - individually, simultaneously or as a group – with the assurance that every PCR cycler provides excellent block homogeneity, accuracy and precision. For security, simply choose different levels of access for different users. Low noise and power usage, and the versatile flexlid® concept for maximized consumable flexibilty, make the MC X50 the most powerful and convenient PCR thermocycler set-up.

Advanced PCR Optimization in a One Step


Mastercycler X50 PCR thermocyclers have a 2D-gradient function that means you can simultaneously optimize both the denaturation temperature (bottom to top) and the annealing temperature (left to right) - see the example of β-actin gene PCR optimization.

Higher denaturation temperatures can increase specificity while lower denaturation temperatures can increase yield, so with the MC X50 PCR cyclers, you can quickly determine the conditions for both optimal yield and specificity. This saves PCR optimization time, is a great benefit in low target copy number and GC-rich target applications, and is really useful in troubleshooting non-specific amplification issues.

Mastercycler® X50

Need rapid PCR results?


In the modern lab, fast is essential, not a luxury. PCR thermocyclers that give you quicker results throughout the day and a faster transition to optimal temperatures mean you can get through more work in a shorter time. The Mastercycler X50 PCR cycler heats at 10 °C/s and cools at 5 °C/s, so make the speed of the MC X50 your research advantage.

Mastercycler® X50

Speed is more than just the ramp rate


There is a wide range of PCR thermocyclers available, with differing claims about ramp rates. However, if you compare the same protocol using different PCR cyclers, you see that ramp rates don’t tell the full story about run time speed. At Eppendorf, we don’t just make claims, we back them up; so we’ve done the performance comparison which shows that the Mastercycler X50 really does give you reliable ramp rates and real PCR speed. To see the full report, see Application Note 274.

Mastercycler® X50

Improving ergonomics across the lab


Ergonomics goes far beyond just the »ergonomically designed chair«. At Eppendorf, we began optimizing our laboratory devices for ergonomics in the early 1970s.

In 2003, we launched the PhysioCare Concept®, focusing on ergonomic liquid handling devices, like our pipettes.

Nowadays, the Eppendorf PhysioCare Concept has broadened to include many laboratory products, including PCR cyclers. Our aim is to offer a holistic solution for harmonizing the workflow in your laboratory with your health and well-being.

For the Eppendorf Mastercyler range, this means:
› Ergonomically designed lid closure
› Low noise level for convenient working conditions at the bench
› Small footprint to enable position in direct arm reach at your bench
› Standardized »user-interface concept« for intuitive handling of devices
› Guided software for easy handling

Further information about the Eppendorf PhysioCare Concept:
www.eppendorf.com/physiocare/

Mastercycler® X50

Simple, intuitive documentation and tracking


The eLABJournal® Electronic Lab Notebook offers an intuitive and flexible solution to document research and track research data, including optimal PCR cycler temperatures and results. Improve efficiency when documenting, organizing, searching, and archiving collected data. With the free eLABJournal add-ons, you can extend the functionality of eLABJournal to a fully integrated Laboratory Information Management System.

Start a free 30-day trial!
Visit www.eLABJournal.com

Mastercycler® X50

Features

  • Heating rate: up to 5 °C/s
  • Wide selection of blocks from a fast silver block to 384 well block
  • VisioNize touchscreen interface
  • Innovative 2D-Gradient for advanced PCR optimization
  • X50 with Touchscreen can control up to 9 other units (connected cyclers do not have a touch screen interface)
  • flexlid® concept: automatic height adjustment of the lid allows you to use all types of consumables
  • Small footprint
  • 2 Year warranty

Applications

  • Fast PCR
  • PCR optimization
  • Standard PCR
  • Incubation
  • Cycle sequencing
 MC X50sMC X50aMC X50pMC X50hMC X50iMC X50lMC X50rMC X50t
Touchscreen Interface
Unit can control 9 other units in network
Unit can only be controlled
ThermoblockSilverAluminumAluminumAluminumSilverAluminumAluminumAluminum
Sample capacity96-well plate or 0.1/0.2 mL tubes96-well plate or 0.1/0.2 mL tubes1 PCR plate 961 PCR plate 38496-well plate or 0.1/0.2 mL tubes96-well plate or 0.1/0.2 mL tubes1 PCR plate 961 PCR plate 384
96-well plate
384-well plate
0.1/0.2 mL tubes
Heating technologySix peltier elementsSix peltier elementsSix peltier elementsSix peltier elementsSix peltier elementsSix peltier elementsSix peltier elementsSix peltier elements
2D-Gradient blockover 12 columns/over 8 rowsover 12 columns/over 8 rowsover 12 columns/over 8 rowsover 24 columns/over 16 rowsover 12 columns/over 8 rowsover 12 columns/over 8 rowsover 12 columns/over 8 rowsover 24 columns/over 16 rows
Temperature control modeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, Safe
Temperature control range of the block4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C
Gradient range1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C
Gradient temperature control range30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C
Block homogeneity 20 – 72 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C
Block homogeneity 95 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C
Control accuracy±0.15 °C±0.15 °C±0.15 °C±0.15 °C±0.15 °C±0.15 °C±0.15 °C±0.15 °C
Cooling rate5 °C/s2.3 °C/s2.3 °C/s2.3 °C/s5 °C/s2.3 °C/s2.3 °C/s2.3 °C/s
Lid descentflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSP
High pressure lid
Lid temperature range37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C
InterfacesEthernet, USBEthernet, USBEthernet, USBEthernet, USBEthernet, USBEthernet, USBEthernet, USBEthernet, USB
Power supply110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz
Max. power consumption850 W850 W850 W850 W850 W850 W850 W850 W
Total weight11.5 kg11.5 kg11.5 kg11.5 kg10.7 kg10.7 kg10.7 kg10.7 kg
Dimensions (W × D × H)27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in
Eppendorf ThermoMixer®        
Heating rate10 °C/s5 °C/s5 °C/s5 °C/s10 °C/s5 °C/s5 °C/s5 °C/s

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