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Microscope Maintenance – Routine Care and Adjustment Procedures

Let Mason Technology help you to get the most out of your Olympus microscope

Your microscope is a high-precision device that will continue to deliver exceptional results if carefully maintained.

To help you with this, our trusted partners at Olympus have put together some free guides on Microscope Maintenance.  Following these routine care-taking procedures will ensure that you continue to get high-quality images from your Olympus microscope.  Whether you are working in an imaging core facility, doing research or educating students – you will always benefit from higher-quality data and fewer sources of error when using a carefully maintained microscope.

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Microscope Technical Training Series

In addition to the guides, we have created a series of 8 short videos to give microscope users a quick visual best practice guide for the alignment, operation, purchasing and maintenance of a microscope.

Answering some of the questions most commonly asked of our microscopy specialists, we cover topics such as  parfocality alignment, köhler illumination, ergonomics, light intensity management, measurement options, objective cleaning, understanding the symbols, and polarisation.

Click here to check out the videos

Imaging Troubleshooting: 3-Minute Microscope Maintenance Check

Are you trying to get an image but the screen is dark?

Follow this step-by-step troubleshooting guide to try and identify errors or incorrect settings that may be causing your imaging problems.

Microscope Cleaning and Maintenance

Dust and dirt particles can accumulate on the microscope and may affect image quality. This is particularly frustrating if dust particles are visible when documenting important specimens that cannot be reused for imaging. Therefore, to ensure you can exploit the full optical quality of your microscope, cleaning should be a regular part of the maintenance routine.

The following notes lead you through the basic procedures – from knowing how to clean the external frame to cleaning the optical system.

Cleaning and Maintenance

Adjusting your Köhler Illumination for Optimum Specimen Illumination

Another important procedure that should be included in your regular maintenance is checking the Koehler illumination.

Koehler Illumination is a method of optical alignment that helps you to produce an evenly illuminated visual field with excellent contrast and maximum sample detail, thus allowing you to exploit the microscope’s full potential.

Köhler Adjustment – Transmitted Light Microscopy

Adjusting the Parfocality of a Camera Adaptor

If you are using a camera, you will need to adapt the focus and adjust the parfocality when first installed.  Over time, if you encounter focus differences between the eyepiece view and the camera image you may need to adjust the parfocality.

Adjusting the Parfocality of a Camera Adaptor

Adjusting For Different Observation Methods

Different observation methods will require the microscope to be adjusted to ensure you are getting the best quality image available.
Our guide explains how to properly adjust the microscope according to your observation method. We offer advice on Differential Interference Contrast (DIC), Phase Contrast (PC), Relief Contrast (RC), and Simple Polarization (PO).

Phase Contrast Adjustment

Get the complete Microscope Maintenance Guide

Interested in hearing more?

If you would like to talk to one of our technical sales consultants on this or any other microscopy queries, fill in the form below and we will get back to you as soon as possible.

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