MALDI-TOF Mass Spectrometry

MALDI-8020 | MALDI Benchtop Instrument

The MALDI-8020 is the latest in a long line of MALDI-TOF products from Shimadzu. This benchtop, linear MALDI-TOF mass spectrometer delivers outstanding performance in a compact footprint, making it an ideal choice for today’s increasingly demanding laboratories.

Linear mode instruments have traditionally been the instruments of choice in MALDI-TOF-based quality control (QC) and profiling workflows, and the MALDI-8020 is no exception. Peptides, proteins, polymers or oligonucleotides – the instrument is capable of delivering the performance required for QC applications. Research laboratories will also benefit from the MS capabilities of the instrument through the rapid mass-measurement of samples.

MALDI-8020 overview

• Linear mode (positive ion) MALDI-TOF
• 200 Hz solid-state laser, 355 nm
• Load-lock chamber for fast sample introduction
• UV laser-based source cleaning (patented)
• Small footprint/benchtop design
• Quiet operation (<55 dB)

MALDI-8020 features

• Sample target solutions: Compatible with the FlexiMass™ series of microscope slide-format sample targets, these slides provide researchers with options depending on their experimental workflow. The individually barcoded, single-use FlexiMass™-DS slides provide a convenient solution for more routine or defined workflows. Ready-to-use, these disposable targets eliminate the need for cleaning and the risk of carryover. Alternatively, the reusable stainless steel FlexiMass™-SR sample targets provide a cost-effective, longer-term solution to sample preparation.
• TrueClean™ automated source cleaning: To maintain instrument performance over time, the MALDI-8020 features wide-bore ion optics – a feature used on our existing MALDI-TOF products – which minimize the risk of source contamination over time, providing a robust platform. To maximize uptime, the system is equipped with TrueClean – an automated, rapid (<10 min) UV laser-based source cleaning which can be used to clean the extraction electrode in-situ without breaking instrument vacuum.
• Facilitates 21 CFR Part 11 compliance: Operating under the control of MALDI Solutions™ software, the software features a centralized, secure Microsoft® SQL database which can be used to store everything from sample lists and acquisition Parameter Sets to acquired MALDI data. The system is managed by an Administrator and customizable user profiles provide control over access to the database and operation of the instrument. Along with a full audit trail, the software provides the tools to help achieve 21 CFR part 11 regulatory compliance.
• Quality as standard: As mass spectrometry moves into clinical laboratories, the MALDI-8020 is the perfect choice for researchers developing MALDI-based diagnostic methods*. The system is designed and manufactured to ISO 9001:2015 and ISO 13485:2016 quality management standards and is CE compliant. Through the selection of components demonstrating improved longevity and performance, and by reducing the number of component parts, our engineers have created a reliable and robust platform capable of delivering outstanding performance in a small footprint. The MALDI systems are maintained by trained service support engineers who form part of the Shimadzu global distribution and support network. Routine maintenance is simplified thanks to the instrument design, providing our engineers with easy access to commonly serviced parts.

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AXIMA Assurance | MALDI Floor Standing Instrument

The Shimadzu AXIMA Assurance a Linear MALDI-TOF mass spectrometry for reliable mass information, an affordable, robust option for all laboratories requiring routine manual or automated analysis of a wide variety of sample classes.

AXIMA Assurance overview

• Linear MALDI-TOF mass spectrometry for reliable mass information.
• An affordable, robust option for all laboratories requiring routine manual or automated analysis of a wide variety of sample classes.
• High sensitivity system using a variable repetition rate 50 Hz N2 laser and a variety of target formats to meet all sample throughput requirements.

AXIMA Assurance features

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MALDI-7090 | Multi-user high-performance MALDI TOF-TOF Mass Spectrometer

The Shimadzu MALDI-7090 is targeted for proteomics and tissue imaging. It combines Shimadzu’s extensive MALDI TOF-TOF mass spectrometry expertise with novel patented technology to provide ultimate performance in identification and structural characterization of biomolecules.

MALDI-7090 overview

• Market leading MS/MS resolution
• Proprietary solid state UV laser technology
• Ultra Fast acquisition speed in MS and MS/MS
• Integrated 10 plate loader
• Newly designed MALDI Solutions™ software

Multi-user high-performance MALDI TOF Mass Spectrometer application areas

MALDI Solutions™ is the completely new software interface for the MALDI-7090 MALDI TOF-TOF mass spectrometer. Providing a powerful range of tools for method development, acquisition, data processing and interpretation, MALDI Solutions has been designed for ease of use and reliability. Each function within the MALDI Solutions suite is targeted to a particular application such as LC, MALDI, MALDI imaging or manual control, creating a simple and versatile application-centric platform.

MALDI-7090 features

• MultiPlex: MultiPlex maximizes the efficient use of the MALDI-7090 through the combination of the integrated 10 plate loader, the 2 kHz ultra fast UV laser and the MALDI Solutions™ multi-user environment software.
• A source design like no other: The MALDI-7090 includes a number of proprietary features to improve sensitivity and productivity.
  • Wide Bore ion Optics: Consisting of unique wide-bore extraction optics, the MALDI-7090 ion optics are designed to maximize ion transmission and minimize source contamination over time.
  • Ultra fast Laser: Shimadzu’s proprietary solid-state UV laser, designed specifically for the MALDI-7090, delivers true 2kHz acquisition speed in all modes. The added benefits of variable beam focus and long lifetime maximize reliability and broad-range applicability.
  • HD sample viewing: It incorporates the latest state-of-the -art camera technology to deliver high-quality full HD color images of the sample with 10µm viewing resolution, software-controlled variable focus and long-life LED illumination.
  • TrueClean™: Optimal performance of the MALDI-7090 source is maintained using TrueClean – a novel and fully automated source cleaning process. TrueClean cleans the source electrodes by redirecting the UV laser onto their surface and desorbing any contaminant material. This method is highly efficient and does not require source removal or venting.
• High-resolution ion gate: It is equipped with a dual wire-grid high resolution ion gate. Compounds of similar nominal mass may produce MS/MS spectra that contain fragment ions from several precursors if not gated correctly. However, the high-resolution ion gate in the MALDI-7090 allows the individual gating of species close in nominal mass thus producing distinct fragment ion spectra.
• Axial Spatial Distribution Focusing – ASDF: ASDF is a Shimadzu patented technology that enables unparalleled resolution in MS/MS acquisitions. Through correction of the axial spatial distribution of the ions generated, the mass resolution is significantly increased and becomes essentially independent of the laser power used to ionize the sample.
• High- and low-energy fragmentation:
  • High-energy CID: MS/MS fragmentation in the MALDI-7090 is true high-energy CID (HE-CID).
  • Low-energy fragmentation (metastable decay): It can also be used in low-energy fragmentation mode without collision gas. Here the fragmentation relies on spontaneous metastable decay of the precursor and exhibits low-energy type ions.

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MALDImini-1 | Digital Ion Trap Mass Spectrometer

Despite its light and compact shape, the MALDImini-1 from Shimadzu allows high-sensitivity MSn measurements, making it suitable for a large number of applications.

Key features of the MALDImini-1 

• “Minimum” footprint: Until now, devices for MSn have had strenuous installation requirements, but the MALDImini-1’s compact size and power requirements make it easy to install almost anywhere. Easy access to the instrument allows users to quickly acquire data and check their results, creating a more convenient workfow right on the bench.
• “Minimum” time: MALDI lends itself to quick analysis through simple sample preparation and acquisition. Once the sample is loaded, users can acquire a spectrum in a few minutes at the click of a button. With such a smooth and quick setup, it is possible to carry out detailed structural analyses in a very short time span.
• “Minimum” sample volume: By combining a MALDI ion source with Digital Ion Trap (DIT) technology, it is possible to carry out high-sensitivity MS and MSn analysis even on micro-quantity samples. Not only can the mass of various molecules be checked, but it is also possible to carry out a wide range of analyses such as identifying proteins and investigating the structure of glycans and glycopeptides.

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AXIMA Confidence | MALDI Floor Standing Instrument

AXIMA Confidence overview

• The Shimadzu AXIMA Confidence has powerful MALDI-TOF performance for reliable mass information and MS/MS derived structural detail.
• Linear mode allows the interrogation of high molecular weight samples, whilst reflectron mode, incorporating the patented curved-field reflectron (CFR), provides the high resolution and mass accuracy necessary for successful proteomics and life science experiments.
• High sensitivity system using a variable repetition rate 50 Hz N2 laser and a variety of target formats to meet all sample throughput requirements.
• An affordable robust option for all laboratories requiring routine manual or automated analysis of a wide variety of sample classes.
• Excellent sensitivity is achieved using near-axis laser irradiation and advanced ion optics for enhanced ion transmission.
• MS/MS may be easily performed using a seamless approach – ions of interest can be isolated using a precursor ion selection device, incorporated as standard, and data-rich fragment ion spectra quickly and simply acquired.The newly improved curved field reflectron design augments the low mass fragment region providing useful additional information.

Shimadzu AXIMA Confidence features and benefits

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