Temperature Control & Mixing

Eppendorf ThermoMixer F

These products are the perfect choice for mixing and heating established routine applications. The Eppendorf ThermoMixer F (F0.5/F1.5/F2.0) and FP have a fixed block for 0.5/1.5/2.0 mL tubes and plates, respectively. Reliable prevention of condensate formation in small vessels can be achieved with the heated lid Eppendorf ThermoTop (optional).

Features of the ThermoMixer F

• Heating and mixing in 0.5/1.5/2.0 mL reaction vessels (F0.5/F1.5/F2.0) or plates (FP)
• Up to 1,500 rpm (F1.5/F2.0) or 2,000 rpm (F0.5/FP) for efficient sample mixing
• Outstanding ^2DMix-Control technology results in excellent mixing performance
• Anti-spill technology prevents lid wetting and cross-contamination
• Compatible with Eppendorf ThermoTop (condens.protect^®) for condensation-free sample handling
• Predefined temperature keys (37 °C, 42 °C, 56 °C, and 95 °C) for simple and intuitive operation
• Very fast heat up speed of up to 18 °C/min, reducing your waiting time
• Eppendorf PhysioCare Concept^® provides ergonomic design and operation

Applications of the ThermoMixer F

• Growth of bacteria and yeasts
• Enzymatic reactions
• Transformation of bacteria strains and plasmids
• Denaturation of DNA, RNA, and proteins
• Lysis reactions at 100 °C
• Labeling of nucleic acids and proteins
• Proteinase K digestion of cells and tissues
• Resuspension of pellets
• Mixing protein quantification assays
• ELISA assays

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Eppendorf ThermoMixer C

The Eppendorf ThermoMixer C combines excellent mixing performance with excellent temperature control to ensure complete, dependable and reproducible test results. Improve your assay results by mixing and incubating samples at the same time. The Eppendorf ThermoMixer C convinces users with its quick and easy-to-exchange SmartBlocks for all common lab vessels, new and improved program functions, and high level of stability for every mixing frequency. There is almost no limit to the variety of applications waiting for you!

Features of the ThermoMixer C

• Mixing, heating, and cooling in one instrument for high flexibility
• All common vessels and plate formats from 5 µL to 50 mL by different Eppendorf SmartBlocks for standard applications
• Ideal for cell thawing when using Eppendorf SmartBlock cryo thaw
• Anti-spill technology prevents lid wetting and cross-contamination
• Increased mixing frequency of up to 3,000 rpm for effective sample mixing
• Excellent temperature management for maximum temperature accuracy, resulting in safe handling
• Compatible with Eppendorf ThermoTop^® (condens.protect^®) for condensation-free sample handling (for Eppendorf SmartBlocks up to 2.0 mL)
• Predefined program keys and clearly arranged menu guides enable simple and intuitive operations
• Freely programmable program keys for individual optimization
• Eppendorf PhysioCare Concept^® provides ergonomic design and operation

Applications of the ThermoMixer C

• Plasmid/RNA/DNA purification
• cDNA synthesis
• Extraction of DNA fragments from agarose gels
• Enzymatic reactions (e.g., DNA restriction digestion, Proteinase K digestion and ligation)
• Transformation of bacteria strains and plasmids
• Denaturation of DNA, RNA, and proteins
• Labeling of DNA, RNA, and proteins
• Bacterial growth in microreaction vessels, conical tubes, and Deepwell Plates
• Lysis reactions at 100 °C
• Mixing of PCR setups

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Eppendorf ThermoTop®

The Eppendorf ThermoTop with condens.protect technology heated lid avoids condensation on tube lid and tube wall. It is compatible with ThermoMixer C/ ThermoStat C with SmartBlock plates and lab vessels up to 2 mL and ThermoMixer F 0.5/F1.5/F2.0/FP.

Tired of condensation in your tubes?

Eppendorf combined the Eppendorf ThermoMixer^® with the PCR cycler approach of a heated lid and generated the Eppendorf ThermoTop. The outstanding Eppendorf condens.protect^® technology reliably prevents condensation within the tube lid – sample concentrations are consistent for the incubation time. In addition, the temperature homogeneity is further improved. Get optimal sample reaction conditions to produce optimal results.

Perfect condensation prevention when dealing with small volumes: The Eppendorf ThermoTop with condens.protect technology heated lid avoids condensation on tube lid and tube wall. It is compatible with ThermoMixer C/ ThermoStat C with SmartBlock plates and lab vessels up to 2 mL and ThermoMixer F 0.5/F1.5/F2.0/FP.

Features of the ThermoTop

Applications ThermoTop

• All temperature control procedures for which the formation of condensation on the tube lid and wall should be reliably avoided

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Eppendorf ThermoStat C

The Eppendorf ThermoStat C is for heating and cooling almost any of your lab vessels. For additional condensation prevention of small volumes we recommend the Eppendorf ThermoTop (optional), a heated lid with condens.protect technology.

Features of the Eppendorf ThermoStat C

• Heating and active cooling in all common vessel and plate formats from 5 µL to 50 mL for high flexibility
• A broad range of Eppendorf SmartBlocks enables high vessel flexibility
• Excellent temperature accuracy for safe sample handling
• Very fast cooling rates of up to 5 °C/min, reducing your waiting time
• Low temperature (e.g. 4 °C or 0 °C) even at higher ambient temperatures for safe sample incubation
• Temperature keys (4 °C, 16 °C, 37 °C, 56 °C, and 95 °C) provide quick access to commonly used experimental temperatures
• Eppendorf ThermoTop^® (condens.protect^®) compatibility prevents condensation and improves temperature homogeneity within sample tubes (up to 2.0 mL)
• A comprehensive range of program slots (up to 15) for flexible usage of the device

ThermoStat C applications

• Gentle thawing and cooling buffers and samples at low temperatures
• Preheating media
• Biochemical reactions at low temperatures
• Ligation
• cDNA synthesis
• Denaturation of RNA, DNA, and protein
• Proteinase K digestion
• ELISA assays
• Immunoprecipitation
• Cell lysis

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Eppendorf MixMate

The Eppendorf MixMate is a 3-in-1 mixer for plates, tubes, and vortex mixing. This high-speed instrument provides complete mixing of your samples in seconds. Outstanding ^2DMix-Control enables optimized mixing with anti-spill technology.

Features of the Eppendorf MixMate

• High-speed mixer (3,000 rpm) for fast and efficient mixing of small volumes in all common vessel formats up to 50 mL
• ^2DMix-Control technology (planar mixing orbit without vertical movement) for controlled mixing and reproducible results in seconds
• Anti-spill technology for controlled mixing movements without lid wetting and cross-contamination
• 5 different tube holders (0.5 mL, 1.5/2.0 mL, 5/15 mL, 30/50 mL, PCR 96-well plates) for high vessel flexibility
• Integrated ergonomic vortex function for various vessel formats for distinct sample mixing
• Minimal hand/arm vibration transfer during vortexing (certificate available) for ergonomic operation (PhysioCare Concept^®)
• 5 soft keys with pre-defined standard mixing parameters for easy usage
• Very stable – remains stationary even at maximum mixing speed
• Whisper quiet operation to benefit the work environment

MixMate applications

• Resuspending pellets (e.g., bacteria, DNA, cell culture pellets)
• Mixing PCR setups
• Mixing viscous liquids and suspensions (incl. beads)
• Mixing restriction digestions
• Immunoassays (e.g., ELISA)
• Colorimetric assays (e.g., Bradford, Lowry, BCA)
• Reporter gene assays (e.g. β-galactosidase, luciferase)
• Vortexing numerous tube formats (e.g., 15 and 50 mL conical tubes)

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