Mastercycler nexus X2 – PCR Thermal Cycler

The Mastercycler^® nexus X2 is Eppendorf‘s solution for busy PCR labs. Two totally independent protocols can be run on the two blocks, so two PCR users can independently start and end their PCR reactions whenever they want. One of the assays can even have up to 64 samples. With less than 40 dB[a] noise emission, the nexus PCR cycler is whisper quiet and also very energy efficient.

Run two independent protocols simultaneously on one PCR thermal cycler with the Eppendorf Mastercycler nexus X2. The 32-well block is well suited for small assays, whereas larger assays can be run on the 64-well block, which is available with a gradient function.

Like every member of the Mastercycler nexus family, the Mastercycler nexus X2 can be combined with all other family members in a network of up to three units. In combination with Eppendorf’s PCR tubes, PCR strips or divisible plates, the Mastercycler nexus X2 will give you consistent, publication-ready results

Mastercycler nexus X2 overview

• The flexlid^® concept for effortless height adjustment: The flexlid concept enables the nexus X2 PCR thermal cycler to deal with all types of consumables, from low volume all the way up to 0.5 mL PCR tubes. Of course, all common PCR plates fit – and if you ever need higher throughput, you can simply connect one or two Mastercycler nexus X2 eco units to your Mastercycler nexus.
• Gradient with SteadySlope^® technology: The Mastercycler pro’s gradient option features SteadySlope technology to ensure that heating and cooling ramp rates are identical in both gradient and normal operation. This gives you identical temperature control characteristics in both optimization and routine experiments. Programming the gradient with the Control Panel and the CycleManager pro is easy and intuitive, so that new users can get started quickly and safely.
• Certified quality: All Eppendorf cyclers follow these quality guidelines:
  • Individual quality control certificates
  • Calibration according to national and international standards, e.g. NIST (USA), DKD/PTB (Germany), UKAS/NPL (UK)
  • UL/cUL listed

Features of the Eppendorf Mastercycler nexus X2

• Asymmetrically divided block for running different assays in parallel, one 32-well and one 64-well block
• Intuitive graphic programming
• Universal block for strips, 0.1 mL, 0.2 mL and 0.5 mL PCR tubes and fits for divisible PCR plates
• Small footprint
• Optional gradient for PCR optimization
• E-mail notification
• flexlid^® concept allows use of all types of consumables with automatic height adjustment of the lid

PCR Thermal Cycler applications

• Standard PCR
• PCR optimization