Liquid Chromatography

• Higher resolution: The analysis time can be effectively shortened because an excellent chromatographic resolution can be maintained even at higher flow rates than those typically used for HPLC.
• Available for both UHPLC and SFC analysis using a single system, Nexera UC/s: Separation results can be quickly obtained during method development by screening with both SFC and UHPLC.
• Small volume fractionation: By using a fraction collector, small-volume fractionation is also possible (ex:1.5 mL vials).
• Fully automated on-line extraction and separation: Target compounds are extracted from solid samples, and then automatically transferred to SFC.
• Nexera UC reduces environmental impact: By using SFC, the consumption of organic solvent is reduced by 94.2%, compared to the normal-phase.

• Unique technology achieves higher recovery rates: SFC shortens analysis times, high recovery rates, stacked injection function eliminates waiting time and simple post-run processing.
• Solid autosamplers for increasing loading capacity: By using SFE, broadening of peak shape at loading a large volume is suppressed.
• Prep Solutions enables a seamless preparative workflow: By using SFE, broadening of peak shape at loading a large volume is suppressed.
• Compact, benchtop design: Benchtop system that can be installed anywhere and wide range of flow rates available.
• Fully automated on-line extraction and fractionation: Target compounds are extracted from solid samples, and then automatically transferred to SFC.
• Nexera UC reduces environmental impact: By using SFC, the consumption of organic solvent is reduced by 94.2%, compared with the normal-phase HPLC.

Nexera Prep overview

• Easy optimization of preparative parameters and scale up
  Fractionation simulation for rapid setup of collection logic Column line-up for scale-up from analytical to preparative
• Time- and energy-saving by automation of the purification workflow
  Collection of target components at high purity by automated desalting
• Expandable to suit the sample/fraction number and volume
  Choose from a wide range of options for recovery scale and analytical detection Problems are resolved simply, to accommodate a variety of needs.

• Streamline and Simplify Establishing the Conditions for Preparative Work: Using the Nexera™ Prep system saves on labor when scaling up from the development of analytical conditions to the conditions for preparative work.
• Preparative Work for Target Components at High Purity Levels and High Concentrations: The Shimadzu UFPLC, Ultra Fast Preparative and Purification Liquid Chromatograph significantly reduces the cost and labor involved in preparative purification. Additionally, the system not only performs purification of target components, but can also recover impurities with high yield enabling direct impurity analysis.
• Preparative Work for Non-UV Absorptive Components: With LH-40 and FRC-40 able to perform signal-based logic and collection on up to four signal channels, not having a chromophore is not a limitation. Nexera Prep can use LCMS, RID, and ELSD to detect and/or identify targets for purification.
• Increased Efficiency from Preparative Analysis Setup to Data Processing: Open Solution is open access software that not only streamlines preparative purification operations, but also supports multi-user operation of preparative systems.
• High Separation via Preparative Recycling: By repeatedly cycling the sample through the column, the target component can be resolved and recovered from coeluting species or impurities without the need for longer or multiple columns.
• Excellent System Expandability: The solvent delivery unit and fraction collector can be selected to suit the recovery volume. Sample introduction and reinjection options cover a wide range of uses. Additionally, the Shim-pack™ Scepter columns feature excellent scalability from analytical to preparative separations with a variety of phases for different applications.

• Double sample throughput with the same method: The Nexera™ MX Dual Stream Technology (MXDST) uses the existing method as is and enables processing twice the number of samples in the same time it takes for conventional systems.
• Quick and automatic optimization of MRM conditions: The newly developed LabSolutions Connect ™ improves the efficiency of required pre-analysis tasks, such as MRM optimization, method creation, and batch creation.
• Simple Workflow Supports High Throughput: Nexera MX simplifies real-time batch analysis and provides an excellent judgment function that enables effective screening of a large number of analytical results obtained from continuous multiplex analysis.

• Intuitive to operate: Hardware and software advances in HPLC over recent years have led to a fully-automated analysis cycle as standard. However, column installation has remained a manual step requiring user know-how. With the LC-2030C NT we introduce a dedicated slide-in column, the Shim-pack NT-ODS. The user can simply insert this column into the front slot and it will be automatically incorporated into the flow path. Analysis can then be begun from the LCD touch panel. The whole procedure can be carried out by staff without specific training. Equipment status and chromatograms can be monitored from outside the lab during analysis, and data results can be processed remotely through a networked PC, opening up more possibilities for flexible workplace.
• Consistent data between users: Column connections can affect the shape of chromatograms, either through small differences between users or human error. Since the Shim-pack NT-ODS is connected automatically by the instrument, there is no need to worry about these possible effects.
• Stable column performance over a large number of samples: The dedicated Shim-pack NT-ODS is a monolithic-type column. The packing state of its separation medium is maintained over a long continuous analysis, whereas a particle-type column may deteriorate. The Shim-pack NT-ODS therefore provides long-term stability even over a large number of continuous injections.

Improved Detection Limits with Microflow

• High Bioanalytical Sensitivity: Sensitivity remains a challenge for many vitamin D metabolites, such as 1,25(OH)2 di-hydroxyvitamin D and 24,25(OH)2 di-hydroxyvitamin D, because they lack easily ionizable functional groups and are in low-abundance. As shown below, microflow LC/MS/MS analysis of standard solutions of the aforementioned vitamin D analytes resulted in a three- to five-fold improvement in the signal-to-noise ratio when compared to semi-microflow analysis.
• Monoclonal Antibody Bioanalysis – Nexera™ Mikros and nSMOL™ Antibody BA Kit: nSMOL (nano-surface and molecular orientation limited proteolysis) is a proprietary, ground-breaking process that enables selective proteolysis of the Fab region of antibodies using trypsin-immobilized nanoparticles. Fab-derived peptides are quantified via MRM measurement on a high-sensitivity mass spectrometer. The Nexera Mikros system achieved signal intensities 12 times higher than with conventional flow rates, with a corresponding lower limit of detection of 0.025 μg/mL and good linearity. The Nexera Mikros system is ideal for low-level quantitation of peptides by LCMS.

Incomparable Microflow Ruggedness

• Realize the Benefits of Microflow While Enjoying the Ruggedness and Reliability of HPLC: The Nexera Mikros system delivers the improvements you expect from a low-flow system with the ruggedness of HPLC. A plasma blank was protein precipitated, diluted (3x), and injected 1,500 times consecutively. During the consecutive injections, QC sample (40 ppt of nortriptyline) was injected once every 50 injections of plasma blank. Peak areas and retention times of QC sample are plotted below. With 4.38% RSD area repeatability and 0.25% RSD retention time stability, extremely stable operation was demonstrated, without loss of peak shape common in high-throughput analyses of complex matrices.

Easy, Familiar Operation

• Micro-ESI™ Sources: Shimadzu’s Micro-ESI LCMS source has been designed for optimal sensitivity and ease of use. An X–Y adjustable stage and viewing camera allow for easy ESI spray needle adjustment. The column oven is mounted on the LCMS to minimize the volume between the exit of the column and the ESI needle.
  The Micro-ESI probe also features an ESI spray angle optimized for microflow rates, and is available for triple quadrupole mass detection mass spectrometryand quadrupole time-of-flight mass spectrometry, respectively.
• CTO-Mikros™ + UF-Link™: Easy, Dependable Column Installation: Mounted to the Micro-ESI source is the CTO-Mikros column oven. Stable column temperature, essential to retention time reproducibility, is ensured and columns up to 150 mm in length can be accommodated. To preserve the optimal source adjustment, the CTO-Mikros features an innovative snap-in column locking system, the UF-Link.
• LC-Mikros™: Innovative Microflow Pump Design: Shimadzu LC pumps are the industry standard for long life and high performance. The LC-Mikros solvent delivery pump has completely new direct-drive engineering to deliver micro to semi-micro flow rates ranging from 1 to 500 μL/min. UHPLC system pressure tolerance of 80 MPa (11,600 psi) allows the use of every column type.
• SIL-40C XR: Low-Volume Direct Injection Autosampler: The SIL-40C XR autosampler delivers you excellent accuracy and reproducibility as well as extremely low carry-over. Its injection volume ranges from 0.1 to 50 μL and your precious sample has never wasted due to the total injection method SIL-40C XR adapted.