Anaerobic Workstations

Whitley A20 Workstation

The Don Whitley Scientific A20 Workstation is one of our smallest anaerobic workstations. It is exceptionally gas efficient and offers ideal conditions for handling, incubating, and examining samples without exposure to breathable oxygen. It maintains a sizable work area while supporting 240 x 90mm Petri dishes. And it can accommodate up to 270 plates for anaerobic incubation in an emergency.

The A20 is the ideal transition device from anaerobic jars to a workstation. It maintains the best anaerobic conditions because it has the same precise parameter control as our larger workstations. You can perform tasks inside the workstation and check your plates as frequently as you like without endangering your samples.

Two oval, multifunctional glove ports on this workstation serve as small airlocks and can each transfer 10 x 90mm Petri dishes as a user inserts their arms.

Additionally available is a side-loading letterbox entry system, which offers a simple means of speedily introducing individual samples into the workstation. The touch screen on the A20 gives the user an intuitive interface for simple control and use of the workstation.

This workstation is the modern replacement for the DG250 Workstation, which was been extremely popular among customers. This new model offers cutting-edge features like a touchscreen with full colour, remote access, data download (for traceability), and the option to monitor anaerobic and catalytic conditions.

This workstation can be used for both anaerobic and microaerobic applications simply by changing the gas supplies.

The Whitley A20 Workstation is a versatile and gas-efficient anaerobic workstation, capable of providing precise control over your samples’ growth environment. This model can hold up to 270 90mm Petri dishes and contains two oval multi-functional glove ports for transferring up to 10 plates at each insertion.

It also has a side-loading letterbox system for introducing individual samples, as well as features such as full-colour touch screen, remote access, data download and monitoring of anaerobic and catalyst conditions.

As the successor to the popular DG250 Workstation, the A20 is perfect for those wanting to take the next step up in sample processing and examination without exposure to atmospheric oxygen.

A20 Workstation Features:

• Very compact footprint – takes up very little bench space but has the same precise control of parameters as our larger workstations.
• A colour, touch-screen control panel for ease of use and for visual display of parameters such as temperature and humidity.
• Low gas consumption – the A20 runs from two gasses (anaerobic mixed gas and nitrogen) for economic running costs.
• Ideal for use as a personal workstation that can be moved if required (a bespoke trolley is available).
• Ethernet-enabled for remote access to the touchscreen.
• Includes an automatic commissioning cycle to save you time and resources.
• Additional options include an integrated anaerobic conditions and catalyst monitoring system with a data download facility.
• Cost comparisons undertaken by DWS show that it is significantly cheaper to operate a Whitley Workstation than it is to use jars.

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Whitley A25 Workstation

The Don Whitley Scientific A25 Workstation might be small but it has the same precise control of parameters as our larger workstations, ensuring the very best anaerobic conditions are maintained. It has a capacity of 300 Petri dishes while still leaving more than adequate space to process samples in a strictly controlled anaerobic environment. Unlike with anaerobic jars, you can check your plates regularly and perform tasks inside the workstation without risk to your samples.

This workstation is equipped with instant access ports (but can be specified with oval, sleeved ports if required) and has a built-in, rapid airlock that can accommodate 20 x 90mm Petri dishes or 3 x 500 ml Duran bottles. The airlock allows items to be transferred into the workstation atmosphere in 20 seconds and the doors are interlocked so cannot be opened simultaneously.

The A25 is fitted with a full colour touchscreen providing the user with an intuitive interface to control and operate the workstation, as well as to display parameters. A single plate entry system (letterbox)is available to provide a straightforward way to quickly introduce individual Petri dishes into the workstation. Other options include cable glands, internal power socket, remote access, data logging(for traceability) and the opportunity to have Anaerobic and Catalyst Conditions Monitoring. The A25 can also be used for microaerobic applications simply by changing the gas supplies.

A25 Workstation Features:

• Previously unavailable on a workstation of this size, Whitley Workstations are the only workstations to provide automatic early warnings about the status of the anaerobic atmosphere and catalyst function.
• With a wide selection of options available, the A25 can fulfil the needs of any laboratory requiring a compact anaerobic workstation.
• Very compact footprint – takes up little bench space but has the same precise control of parameters as larger Whitley Hypoxystations.
• The colour touchscreen is controlled for ease of use and for visual display of parameters such as temperature and humidity.
• Ideal for use as a personal workstation that can be moved if required (a bespoke trolley is available).
• Ethernet-enabled for remote access to the touchscreen.

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Whitley A35 Workstation

The Don Whitley Scientific A35 Workstation provides excellent conditions for the processing, incubation and examination of samples without exposure to atmospheric oxygen. This anaerobic cabinet provides the ability to manipulate samples in a sustainable environment where parameters can be altered to create the required conditions. The colour touchscreen panel displays the parameters such as temperature, humidity and airlock cycle status but also provides easy access to control the workstation. A built-in, rapid airlock ensures samples can be transferred into the workstation atmosphere as soon as possible.

The standard A35 Workstation features two oval, sleeved ports on a fixed front. This workstation can also be specified with our unique instant access porthole system that allows entry to the chamber in seconds and needs neither gloves nor sleeves.

Additional options include HEPA filtration, removable front, automatic sleeve gassing, integrated anaerobic conditions and catalyst monitoring system, and data download facility. The optional HEPA model is fitted with the unique Whitley Internal HEPA Filtration System. All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly, providing levels of atmospheric cleanliness inside the workstation that exceed the requirements of ISO 14644 Class 3.

Applications for this workstation include clinical microbiology, dental and research into the human gut microbiome.

With the Whitley A35, sample manipulation within a secure anaerobic environment is as easy as working aerobically at the laboratory bench.

A35 Workstation Features:

• Accommodates up to 600 x 90mm Petri dishes.
• Available with a removable front to facilitate thorough cleaning and the transfer of bulk samples and larger pieces of equipment for use in the workstation.
• Optional single plate entry system for the rapid introduction of Petri dishes.
• Ethernet-enabled for remote access to the touchscreen.
• Chilled incubation system is available for media storage.
• Includes an automatic commissioning cycle to save you time and resources.
• Optional accessories include the Automatic Gas Changeover Unit – never run out of gas again!
• NEW – now available with a motorised height adjustable trolley.

Download the Whitley HEPA Filtration Leaflet

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Whitley A45 Workstation

The Don Whitley Scientific A45 Workstation, like our other anaerobic workstations, enables the processing, incubation and examination of samples without exposure to atmospheric oxygen. This workstation has three ports for convenient user access to the entire incubation and working areas.

The A45 Workstation accommodates up to 780 x 90mm Petri dishes whilst still providing a generous working area. The A45 is available as an A45 HEPA, fitted with the unique Whitley Internal HEPA Filtration System. All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly. Additional options include an integrated anaerobic conditions and catalyst monitoring system with data download facility.

Sample manipulation within a secure anaerobic environment is now as easy as working aerobically at the laboratory bench.

A45 Workstation Features:

• The built-in rapid airlock allows the transfer of plates in just 60 seconds.
• A colour, touchscreen control panel for ease of use and for visual display of parameters such as temperature, humidity, and airlock cycle status.
• Ethernet-enabled for remote access to touchscreen.
• Optional removable front with instant access or traditional sleeved ports.
• Available with a mixture of instant access ports and conventional ports to give you the best arrangement for your application
• Includes an automatic commissioning cycle to save you time and resources.
• Optional accessories include the Automatic Gas Changeover Unit – never run out of gas again.

Download the Whitley HEPA Filtration Leaflet

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Whitley A55 Workstation

The Don Whitley Scientific A55 Workstation provides a huge working capacity when there is a need to easily process, culture and examine samples without exposure to atmospheric oxygen. This workstation has two airlocks (one on the left and one on the right) and provides the ability to manipulate samples in a sustainable environment where parameters can be altered to create the required conditions.

With the A55 Workstation, you have a choice of port configurations; for example, you can have four instant access portholes that allow entry to the chamber in seconds without the need for gloves or sleeves or you can have one set of these ports and one set of sleeved ports.

With two sets of ports, two people can easily work side-by-side in this chamber, in the same environmental conditions. This is ideal for training situations or for labs with high throughput.

The HEPA-filtered version of the A55 is fitted with the unique Whitley Internal HEPA Filtration System. All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly, providing levels of atmospheric cleanliness inside the workstation that exceed the requirements of ISO 14644 Class 3.

Also available with fully integrated anaerobic conditions and catalyst monitoring system with a data download facility.

A55 Options:

• Five different sleeveless/sleeved port configurations.
• Single plate entry system
• Data logging capability
• Internal electrical sockets

If you want to be able to operate your workstation with two different temperature levels, specify the dual temperature option. This provides a central divider between the workstation chambers allowing you to select different temperatures for each side. A manually operated sliding door between the two chambers allows samples to be passed from one area into the other.

Sample manipulation within a secure anaerobic environment is now as easy as working aerobically at the laboratory bench.

A55 Workstation Features:

• • • • Accommodates between 1,080 and 1,200 x 90mm Petri dishes (depending on whether plate carriers are used and which accessories and system options have been incorporated).
      • Removable front as standard on the right-hand half of the unit to facilitate the transfer of bulk samples or larger pieces of equipment for use inside the workstation.
      • Two rapid airlocks each have a 40 Petri dish capacity and allow samples to be introduced and withdrawn at either end of the workstation offering maximum flexibility and improving the workflow in your laboratory. Both airlocks can be operated at the same time.
      • Colour, touchscreen control panel for ease of use and for visual display of parameters such as temperature, humidity, and airlock cycle status.
      • Ethernet-enabled for remote access to the touchscreen.
      • Comes complete with a custom-designed trolley.
      • Optional conventional, oval, sleeved ports or a mixture of oval and instant access ports.
      • Automatic commissioning cycle to save you time and resources.

   

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Whitley A85 Workstation

The Don Whitley Scientific A85 Workstation is used when there is a need to easily process, incubate and examine samples without exposure to atmospheric oxygen. This anaerobic workstation has a large, 36-litre airlock and accommodates up to 560 x 90mm plates.  Oval glove ports enable you to work comfortably, gloved or bare-handed. Each glove port also acts as a mini-airlock so you can transfer up to 10 plates through each as you insert or withdraw your arms.

The colour, touch-screen control panel ensures ease of use and is perfect for visual display of parameters such as temperature, humidity, and airlock cycle status.

The A85 Workstation can be purchased with the Whitley Internal HEPA Filtration System or can include a refrigeration unit should you want to manipulate samples and incubate cultures at low temperatures. This workstation can also be purchased as a three-gas (TG) version, which runs from separate cylinders of nitrogen, hydrogen and carbon dioxide. So if you need to reduce costs to a minimum ask about the A85TG Workstation.

A85 Workstation Features:

• The airlock, with a powered internal door, provides effective sample and equipment transfer in the fastest possible time whilst ensuring the minimum amount of oxygen is introduced into the chamber.
• The dual gas operation minimises running costs or orders the TG version to further reduce costs.
• Includes automatic commissioning cycle to save you time and resources.
• Options include HEPA filtration, a refrigeration unit, and an integrated Anaerobic Conditions Monitor with a data download facility.

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Whitley A95 Workstation

The Don Whitley Scientific A95 Workstation has a huge capacity of between 960 and 1120 x 90mm Petri dishes, depending upon the way you choose to configure your working and incubation areas. Incorporating the latest in touch screen technology, some features and benefits of the A95 are unique to DWS.

The large, integral 36 litre airlock with automatic internal door (no awkward locking mechanism) is 20% faster than the previous MG1000 model. The cycle takes only 5 minutes, which ensures a considerable saving in gas usage. Four manual, oval glove ports enable two people to operate within the same controlled atmosphere simultaneously. This is ideal for tutoring or for very busy laboratories.

The A95 Workstation can be purchased as a three gas (TG) version, which runs from separate cylinders of nitrogen, hydrogen and carbon dioxide. So if you need to reduce costs to a minimum ask about the A95TG. The A95 can also be purchased with the Whitley Internal HEPA Filtration System.

Other options include internal power sockets, microscope attachment and single plate entry systems – to tailor the system to your specific requirements.

A95 Workstation Features:

• Each porthole also acts as a mini-airlock so you can transfer 10 plates through each time you insert or withdraw your arms, so reducing airlock cycles and gas usage.
• With dual gas operation, this workstation is very cost-effective but a TG (three gas) version can also be specified to further reduce costs.
• With the HEPA model, levels of atmospheric cleanliness inside the workstation exceed the requirements of ISO 14644 Class 3. Proof of atmospheric cleanliness can be provided on request.
• Available with fully integrated Anaerobic Conditions Monitor with data download facility.
• Optional automatic sleeve gassing for cost-effective gas usage and to ensure internal conditions are maintained when entering the chamber.

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Whitley A135 HEPA Workstation

The Don Whitley Scientific A135 HEPA Workstation is a tall, wide, anaerobic workstation fitted with a unique internal HEPA filtration system. Like our other anaerobic chambers, it provides excellent conditions for the processing, incubation and examination of samples without exposure to atmospheric oxygen. With an internal volume of 950 litres, this workstation can accommodate a variety of laboratory equipment, eg cell sorters, colony pickers, high throughput bio-reactors, microscopes and spiral platers such as the Whitley WASP Touch.

Fitted with the unique Whitley Internal HEPA Filtration System. All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly. DWS filters are located inside the workstation, which prevents the filter from becoming saturated with moisture and rendered ineffective.

The internal atmosphere is circulated continuously – contributing to even temperature distribution and ensuring any internal particulates will be in motion before capture by the filter. Levels of atmospheric cleanliness inside the workstation exceed the requirements of ISO 14644 Class 3. Proof of atmospheric cleanliness can be provided on request.

As well as providing ideal conditions for anaerobic studies, the A135 HEPA Workstation operates from two gas supplies for the most cost-effective running conditions.

A135 HEPA Workstation Features:

• The built-in rapid airlock ensures samples can be transferred into the workstation atmosphere as soon as possible (takes only 60 seconds).
• Fitted with a removable front to facilitate thorough cleaning, easy replacement of the HEPA filter, and the transfer of bulk samples and larger pieces of equipment for use in the workstation.
• Fully integrated gas control avoids bulky add-on systems – reduces the need for additional bench space.
• A colour, touch-screen control panel for ease of use and for visual display of parameters such as temperature, humidity, and airlock cycle status.
• Includes an automatic commissioning cycle to save you time and resources.
• Options include an integrated Anaerobic Conditions Monitor with a data download facility.

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Whitley A155 HEPA Workstation

With the Don Whitley Scientific A155 HEPA Workstation, you can operate a wide range of laboratory equipment or carry out specialised tasks in a strictly regulated anaerobic environment. This workstation has two airlocks and a 1,800 litre capacity.

Because of its large capacity, this workstation can hold a wide range of laboratory instruments, including spiral platers such as the Whitley WASP Touch, high throughput bio-reactors, cell sorters, and colony pickers. Equipment may be moved into and out of the chamber much more easily thanks to the two removable fronts.

The Whitley Internal HEPA Filtration System on the Whitley A155 HEPA lowers particle counts inside the chamber to levels exceeding ISO 14644 Class 3 criteria. Hundreds of times per hour, the entire atmosphere is passed through the filter, ensuring efficient cleaning of the chamber environment. The DWS filters are located inside the workstation, which stops them from becoming damp and losing their effectiveness.

This anaerobic workstation has two deep airlocks, one at each end, that may be independently operated and complete a cycle in under 60 seconds. Each airlock can hold ten Duran 500ml bottles.

A bespoke trolley with storage is included with the Whitley A155 HEPA.

Whitley A155 HEPA Workstation Features:

• Two removable fronts enable the transfer of larger batches of samples or pieces of equipment for use inside the chamber.
• Patented oval ports enable you to work comfortably, gloved or bare-handed.
• Each port includes an isolator switch so that you can use one port at a time if required. The fact that there are two sets of ports means that two researchers can work in the chamber side-by-side.
• Colour, touch-screen control panel for ease of use and for visual display of parameters such as temperature, humidity, and airlock cycle status.
• Two new options are available: a rotating turntable (up to 3 per shelf) to hold up to 80 x 90mm Petri dishes; and a movable platform to facilitate the movement of heavy items of equipment inside the chamber.
• Other options include a double internal power socket, front loading single sample entry system, cable glands, shelves with optional storage trays, Anaerobic Conditions Monitor and data logging.
• A fully comprehensive maintenance and breakdown contract is also available to prolong the life of your investment. (Note: different arrangements apply in different countries).

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Whitley A135 GMP Anaerobic Workstation

Don Whitley Scientific has over 40 years of experience in the design and manufacture of anaerobic cabinets, and in 2013 developed the Whitley Internal HEPA Filtration System, primarily to serve our research customers. In collaboration with customers running GMP licensed facilities, we have now developed innovative uni-directional airflow technology, and successfully added this feature to an anaerobic workstation to create a unique product offering: a uni-directional airflow workstation that allows users to process samples under strict anaerobic conditions.

The A135 GMP Anaerobic Workstation is specifically designed to be used as a clean-air isolator in processes following Good Manufacturing Practice (GMP). This microbiology isolator provides Grade A air cleanliness (EU GMP), combining sterile uni-directional airflow, positive operating pressure, and physical isolation to provide highly effective product protection. Unlike traditional isolators that use inlet air from the surrounding room, this workstation is a fully closed recirculating system so can be housed in a Grade D cleanroom during GMP compliant use.

Two sizes of airlock are available for this workstation: one is 17.5 litres and provides for the effective transfer of up to 10 x 500ml Duran bottles (or items of a similar size) to and from the chamber in just 2 minutes. The dimensions of this airlock are 197mm x 388mm x 190mm (W x D x H). The larger airlock is 43 litres (measuring 335 x 300 x 300mm) providing effective transfer for items of equipment or for up to 20 x 500ml Duran bottles in just 5 minutes.

With internal working area dimensions of (w x d x h): 1075 x 750 x 608mm, there is a generous amount of working space inside the workstation chamber to house equipment such as centrifuges and homogenisers. The patented oval glove ports provide maximum comfort when used over extended periods.

A135 GMP Anaerobic Workstation Features:

• This Grade A anaerobic isolator maintains strict anaerobic conditions, operating from two gas supplies for the most effective and efficient running costs.
• HEPA filter face velocity/uniformity of uni-directional airflow and Dispersed Oil Particulate (DOP) tests are carried out in accordance with ISO14644-3 to validate system integrity and performance. All tests are performed by an independent organisation.
• The GMP Workstation exceeds the requirements of Grade A air cleanliness (EU GMP) and ISO 14644-1 Class 3 both at rest and in operation.
• Whitley Intelligent Monitoring continually measures atmospheric downflow to ensure optimised filter performance.
• The ‘Standby’ feature (ECO mode) automatically decreases fan speed when the chamber is at rest to maximise energy efficiency.
• The large workstation chamber is perfect for accommodating a variety of items of equipment (removable front fitted as standard).
• A password-protected full-colour touch screen interface allows easy monitoring of all parameters simultaneously. Alarms and status notices are also displayed.
• This workstation is Ethernet-enabled for remote access review and control.
• Data logging system records all monitored parameters every 60 seconds.
• The Event Log lists events showing the date and time they occurred. The log retains information for 100 days and can be downloaded if the Data Logging option has been purchased.
• Air sampling ports can be fitted to allow monitoring systems to be connected to the workstation for continuous particulate monitoring and microbial air sampling.
• A fully automated de-humidification system is included as standard. It does not require any operator intervention.
• A bespoke trolley with heavy-duty castors is provided as standard. A trolley not only saves bench space but is also useful for ease of mobility for service and maintenance.
• Other optional features are available to tailor the system to your requirements.
• The workstation can also be run aerobically if required.
• Range of possible decontamination protocols, including the use of vaporised hydrogen peroxide.

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Whitley A155 GMP Workstation

The Don Whitley Scientific A155 GMP Anaerobic Workstation is specifically designed to be used as a clean-air isolator in processes following Good Manufacturing Practice (GMP). This is the first Whitley GMP Workstation that allows two people to work in the chamber at same time. The A155 GMP Workstation provides Grade A air cleanliness (EU GMP) and combines sterile anaerobic uni-directional airflow, positive operating pressure, and physical isolation to provide highly effective product protection. Unlike traditional isolators that use inlet air from the surrounding room, this workstation is a fully closed recirculating system so can be housed in a Grade D cleanroom during GMP compliant use.

Each workstation is tested to ensure compliance with the relevant portions of ISO 14644-3. This will be of special interest to users conducting anaerobic processes that must comply with GMP requirements. In addition, tests carried out in our own laboratories confirm the integral HEPA filtered uni-directional airflow system meets the requirements set out in BS EN 12469:2000 with regard to protection of the working area from particulate and microbial contamination.

Don Whitley Scientific engineers are trained to carry out filter integrity (DOP) testing on HEPA filters. This allows us to provide full validation on Whitley GMP Anaerobic Workstations. Customers who work to GMP processes can rest assured that they don’t have to source their own external, specialist organisations to perform these important tests once their workstation has been installed. DWS has invested in the relevant equipment and training to provide this one-stop solution. Customers can have the confidence that their workstation has been tested by engineers who are fully trained in the operation and manufacture of Whitley Workstations.

This workstation is equipped with two integral airlocks (one at either side of the main workstation chamber) providing an ideal layout for a ‘production line’ style of working. Two sizes of airlock are available: 17.5 litres or 43 litres. These airlocks have been specifically designed to aid the transfer of serological pipettes into the workstation. Each 17.5 litre airlock is capable of accommodating 10 x 500ml Duran bottles and the internal dimensions are 197mm x 388mm x 190mm (W x D x H). The 43 litre airlocks (measuring 335 x 300 x 300mm) can each accommodate up to 20 x 500ml Duran bottles.

The patented oval glove ports provide maximum comfort when used over extended periods.

With internal working area dimensions of (w x d x h): 2200 x 750 x 620mm, there is a huge amount of working space inside the workstation chamber to house equipment such as centrifuges and homogenisers.

Example applications for this workstation include faecal microbiota transplant; probiotics manufacture; bacteria manufacture; live biotherapeutics; biopharmaceuticals; and microbiome therapeutics.

A155 GMP Workstation Features:

• This workstation maintains strict anaerobic conditions, operating from two gas supplies for the most effective and efficient running costs.
• HEPA filter face velocity/uniformity of uni-directional airflow and Dispersed Oil Particulate (DOP) tests are carried out in accordance with ISO14644-3 to validate system integrity and performance. All tests are performed by an independent organisation.
• Whitley Intelligent Monitoring continually measures atmospheric downflow to ensure optimised filter performance.
• The ‘Standby’ feature (ECO mode) automatically decreases fan speed when the chamber is at rest to maximise energy efficiency.
• The large workstation chamber is perfect for accommodating a variety of items of equipment, which can be installed and removed via the removable front (fitted as standard).
• A password-protected full-colour touch screen interface allows easy monitoring of all parameters simultaneously. Alarms and status notices are also displayed.
• This workstation is Ethernet-enabled for remote access review and control.
• Four oval, sleeved ports and two airlocks allow two people to work in the chamber at the same time.
• Data logging system records all monitored parameters every 60 seconds
• The Event Log lists events showing the date and time they occurred. The log retains information for 100 days and can be downloaded if the Data Logging option has been purchased.
• A fully automated de-humidification system is included as standard. It does not require any operator intervention.
• A bespoke trolley with heavy-duty castors is provided as standard. A trolley not only saves desk space but is also useful for ease of mobility for service and maintenance.
• Other optional features are available to tailor the system to your requirements.
• Workstations can also be run aerobically if required.
• Range of possible decontamination protocols, including the use of vaporised hydrogen peroxide.
• Air sampling ports can be fitted to allow particulate monitoring systems to be connected to the workstation for continuous particulate monitoring.

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