Don Whitley Scientific

Whitley A20 Workstation

The Don Whitley Scientific A20 Workstation is one of our smallest anaerobic workstations. It is exceptionally gas efficient and offers ideal conditions for handling, incubating, and examining samples without exposure to breathable oxygen. It maintains a sizable work area while supporting 240 x 90mm Petri dishes. And it can accommodate up to 270 plates for anaerobic incubation in an emergency.

The A20 is the ideal transition device from anaerobic jars to a workstation. It maintains the best anaerobic conditions because it has the same precise parameter control as our larger workstations. You can perform tasks inside the workstation and check your plates as frequently as you like without endangering your samples.

Two oval, multifunctional glove ports on this workstation serve as small airlocks and can each transfer 10 x 90mm Petri dishes as a user inserts their arms.

Additionally available is a side-loading letterbox entry system, which offers a simple means of speedily introducing individual samples into the workstation. The touch screen on the A20 gives the user an intuitive interface for simple control and use of the workstation.

This workstation is the modern replacement for the DG250 Workstation, which was been extremely popular among customers. This new model offers cutting-edge features like a touchscreen with full colour, remote access, data download (for traceability), and the option to monitor anaerobic and catalytic conditions.

This workstation can be used for both anaerobic and microaerobic applications simply by changing the gas supplies.

The Whitley A20 Workstation is a versatile and gas-efficient anaerobic workstation, capable of providing precise control over your samples’ growth environment. This model can hold up to 270 90mm Petri dishes and contains two oval multi-functional glove ports for transferring up to 10 plates at each insertion.

It also has a side-loading letterbox system for introducing individual samples, as well as features such as full-colour touch screen, remote access, data download and monitoring of anaerobic and catalyst conditions.

As the successor to the popular DG250 Workstation, the A20 is perfect for those wanting to take the next step up in sample processing and examination without exposure to atmospheric oxygen.

A20 Workstation Features:

• Very compact footprint – takes up very little bench space but has the same precise control of parameters as our larger workstations.
• A colour, touch-screen control panel for ease of use and for visual display of parameters such as temperature and humidity.
• Low gas consumption – the A20 runs from two gasses (anaerobic mixed gas and nitrogen) for economic running costs.
• Ideal for use as a personal workstation that can be moved if required (a bespoke trolley is available).
• Ethernet-enabled for remote access to the touchscreen.
• Includes an automatic commissioning cycle to save you time and resources.
• Additional options include an integrated anaerobic conditions and catalyst monitoring system with a data download facility.
• Cost comparisons undertaken by DWS show that it is significantly cheaper to operate a Whitley Workstation than it is to use jars.

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Whitley A25 Workstation

The Don Whitley Scientific A25 Workstation might be small but it has the same precise control of parameters as our larger workstations, ensuring the very best anaerobic conditions are maintained. It has a capacity of 300 Petri dishes while still leaving more than adequate space to process samples in a strictly controlled anaerobic environment. Unlike with anaerobic jars, you can check your plates regularly and perform tasks inside the workstation without risk to your samples.

This workstation is equipped with instant access ports (but can be specified with oval, sleeved ports if required) and has a built-in, rapid airlock that can accommodate 20 x 90mm Petri dishes or 3 x 500 ml Duran bottles. The airlock allows items to be transferred into the workstation atmosphere in 20 seconds and the doors are interlocked so cannot be opened simultaneously.

The A25 is fitted with a full colour touchscreen providing the user with an intuitive interface to control and operate the workstation, as well as to display parameters. A single plate entry system (letterbox)is available to provide a straightforward way to quickly introduce individual Petri dishes into the workstation. Other options include cable glands, internal power socket, remote access, data logging(for traceability) and the opportunity to have Anaerobic and Catalyst Conditions Monitoring. The A25 can also be used for microaerobic applications simply by changing the gas supplies.

A25 Workstation Features:

• Previously unavailable on a workstation of this size, Whitley Workstations are the only workstations to provide automatic early warnings about the status of the anaerobic atmosphere and catalyst function.
• With a wide selection of options available, the A25 can fulfil the needs of any laboratory requiring a compact anaerobic workstation.
• Very compact footprint – takes up little bench space but has the same precise control of parameters as larger Whitley Hypoxystations.
• The colour touchscreen is controlled for ease of use and for visual display of parameters such as temperature and humidity.
• Ideal for use as a personal workstation that can be moved if required (a bespoke trolley is available).
• Ethernet-enabled for remote access to the touchscreen.

Download the Anaerobic Range Brochure

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Whitley A35 Workstation

The Don Whitley Scientific A35 Workstation provides excellent conditions for the processing, incubation and examination of samples without exposure to atmospheric oxygen. This anaerobic cabinet provides the ability to manipulate samples in a sustainable environment where parameters can be altered to create the required conditions. The colour touchscreen panel displays the parameters such as temperature, humidity and airlock cycle status but also provides easy access to control the workstation. A built-in, rapid airlock ensures samples can be transferred into the workstation atmosphere as soon as possible.

The standard A35 Workstation features two oval, sleeved ports on a fixed front. This workstation can also be specified with our unique instant access porthole system that allows entry to the chamber in seconds and needs neither gloves nor sleeves.

Additional options include HEPA filtration, removable front, automatic sleeve gassing, integrated anaerobic conditions and catalyst monitoring system, and data download facility. The optional HEPA model is fitted with the unique Whitley Internal HEPA Filtration System. All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly, providing levels of atmospheric cleanliness inside the workstation that exceed the requirements of ISO 14644 Class 3.

Applications for this workstation include clinical microbiology, dental and research into the human gut microbiome.

With the Whitley A35, sample manipulation within a secure anaerobic environment is as easy as working aerobically at the laboratory bench.

A35 Workstation Features:

• Accommodates up to 600 x 90mm Petri dishes.
• Available with a removable front to facilitate thorough cleaning and the transfer of bulk samples and larger pieces of equipment for use in the workstation.
• Optional single plate entry system for the rapid introduction of Petri dishes.
• Ethernet-enabled for remote access to the touchscreen.
• Chilled incubation system is available for media storage.
• Includes an automatic commissioning cycle to save you time and resources.
• Optional accessories include the Automatic Gas Changeover Unit – never run out of gas again!
• NEW – now available with a motorised height adjustable trolley.

Download the Whitley HEPA Filtration Leaflet

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Whitley A45 Workstation

The Don Whitley Scientific A45 Workstation, like our other anaerobic workstations, enables the processing, incubation and examination of samples without exposure to atmospheric oxygen. This workstation has three ports for convenient user access to the entire incubation and working areas.

The A45 Workstation accommodates up to 780 x 90mm Petri dishes whilst still providing a generous working area. The A45 is available as an A45 HEPA, fitted with the unique Whitley Internal HEPA Filtration System. All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly. Additional options include an integrated anaerobic conditions and catalyst monitoring system with data download facility.

Sample manipulation within a secure anaerobic environment is now as easy as working aerobically at the laboratory bench.

A45 Workstation Features:

• The built-in rapid airlock allows the transfer of plates in just 60 seconds.
• A colour, touchscreen control panel for ease of use and for visual display of parameters such as temperature, humidity, and airlock cycle status.
• Ethernet-enabled for remote access to touchscreen.
• Optional removable front with instant access or traditional sleeved ports.
• Available with a mixture of instant access ports and conventional ports to give you the best arrangement for your application
• Includes an automatic commissioning cycle to save you time and resources.
• Optional accessories include the Automatic Gas Changeover Unit – never run out of gas again.

Download the Whitley HEPA Filtration Leaflet

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Whitley A55 Workstation

The Don Whitley Scientific A55 Workstation provides a huge working capacity when there is a need to easily process, culture and examine samples without exposure to atmospheric oxygen. This workstation has two airlocks (one on the left and one on the right) and provides the ability to manipulate samples in a sustainable environment where parameters can be altered to create the required conditions.

With the A55 Workstation, you have a choice of port configurations; for example, you can have four instant access portholes that allow entry to the chamber in seconds without the need for gloves or sleeves or you can have one set of these ports and one set of sleeved ports.

With two sets of ports, two people can easily work side-by-side in this chamber, in the same environmental conditions. This is ideal for training situations or for labs with high throughput.

The HEPA-filtered version of the A55 is fitted with the unique Whitley Internal HEPA Filtration System. All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly, providing levels of atmospheric cleanliness inside the workstation that exceed the requirements of ISO 14644 Class 3.

Also available with fully integrated anaerobic conditions and catalyst monitoring system with a data download facility.

A55 Options:

• Five different sleeveless/sleeved port configurations.
• Single plate entry system
• Data logging capability
• Internal electrical sockets

If you want to be able to operate your workstation with two different temperature levels, specify the dual temperature option. This provides a central divider between the workstation chambers allowing you to select different temperatures for each side. A manually operated sliding door between the two chambers allows samples to be passed from one area into the other.

Sample manipulation within a secure anaerobic environment is now as easy as working aerobically at the laboratory bench.

A55 Workstation Features:

• • • • Accommodates between 1,080 and 1,200 x 90mm Petri dishes (depending on whether plate carriers are used and which accessories and system options have been incorporated).
      • Removable front as standard on the right-hand half of the unit to facilitate the transfer of bulk samples or larger pieces of equipment for use inside the workstation.
      • Two rapid airlocks each have a 40 Petri dish capacity and allow samples to be introduced and withdrawn at either end of the workstation offering maximum flexibility and improving the workflow in your laboratory. Both airlocks can be operated at the same time.
      • Colour, touchscreen control panel for ease of use and for visual display of parameters such as temperature, humidity, and airlock cycle status.
      • Ethernet-enabled for remote access to the touchscreen.
      • Comes complete with a custom-designed trolley.
      • Optional conventional, oval, sleeved ports or a mixture of oval and instant access ports.
      • Automatic commissioning cycle to save you time and resources.

   

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Whitley A85 Workstation

The Don Whitley Scientific A85 Workstation is used when there is a need to easily process, incubate and examine samples without exposure to atmospheric oxygen. This anaerobic workstation has a large, 36-litre airlock and accommodates up to 560 x 90mm plates.  Oval glove ports enable you to work comfortably, gloved or bare-handed. Each glove port also acts as a mini-airlock so you can transfer up to 10 plates through each as you insert or withdraw your arms.

The colour, touch-screen control panel ensures ease of use and is perfect for visual display of parameters such as temperature, humidity, and airlock cycle status.

The A85 Workstation can be purchased with the Whitley Internal HEPA Filtration System or can include a refrigeration unit should you want to manipulate samples and incubate cultures at low temperatures. This workstation can also be purchased as a three-gas (TG) version, which runs from separate cylinders of nitrogen, hydrogen and carbon dioxide. So if you need to reduce costs to a minimum ask about the A85TG Workstation.

A85 Workstation Features:

• The airlock, with a powered internal door, provides effective sample and equipment transfer in the fastest possible time whilst ensuring the minimum amount of oxygen is introduced into the chamber.
• The dual gas operation minimises running costs or orders the TG version to further reduce costs.
• Includes automatic commissioning cycle to save you time and resources.
• Options include HEPA filtration, a refrigeration unit, and an integrated Anaerobic Conditions Monitor with a data download facility.

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Whitley A95 Workstation

The Don Whitley Scientific A95 Workstation has a huge capacity of between 960 and 1120 x 90mm Petri dishes, depending upon the way you choose to configure your working and incubation areas. Incorporating the latest in touch screen technology, some features and benefits of the A95 are unique to DWS.

The large, integral 36 litre airlock with automatic internal door (no awkward locking mechanism) is 20% faster than the previous MG1000 model. The cycle takes only 5 minutes, which ensures a considerable saving in gas usage. Four manual, oval glove ports enable two people to operate within the same controlled atmosphere simultaneously. This is ideal for tutoring or for very busy laboratories.

The A95 Workstation can be purchased as a three gas (TG) version, which runs from separate cylinders of nitrogen, hydrogen and carbon dioxide. So if you need to reduce costs to a minimum ask about the A95TG. The A95 can also be purchased with the Whitley Internal HEPA Filtration System.

Other options include internal power sockets, microscope attachment and single plate entry systems – to tailor the system to your specific requirements.

A95 Workstation Features:

• Each porthole also acts as a mini-airlock so you can transfer 10 plates through each time you insert or withdraw your arms, so reducing airlock cycles and gas usage.
• With dual gas operation, this workstation is very cost-effective but a TG (three gas) version can also be specified to further reduce costs.
• With the HEPA model, levels of atmospheric cleanliness inside the workstation exceed the requirements of ISO 14644 Class 3. Proof of atmospheric cleanliness can be provided on request.
• Available with fully integrated Anaerobic Conditions Monitor with data download facility.
• Optional automatic sleeve gassing for cost-effective gas usage and to ensure internal conditions are maintained when entering the chamber.

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Whitley A155 HEPA Workstation

With the Don Whitley Scientific A155 HEPA Workstation, you can operate a wide range of laboratory equipment or carry out specialised tasks in a strictly regulated anaerobic environment. This workstation has two airlocks and a 1,800 litre capacity.

Because of its large capacity, this workstation can hold a wide range of laboratory instruments, including spiral platers such as the Whitley WASP Touch, high throughput bio-reactors, cell sorters, and colony pickers. Equipment may be moved into and out of the chamber much more easily thanks to the two removable fronts.

The Whitley Internal HEPA Filtration System on the Whitley A155 HEPA lowers particle counts inside the chamber to levels exceeding ISO 14644 Class 3 criteria. Hundreds of times per hour, the entire atmosphere is passed through the filter, ensuring efficient cleaning of the chamber environment. The DWS filters are located inside the workstation, which stops them from becoming damp and losing their effectiveness.

This anaerobic workstation has two deep airlocks, one at each end, that may be independently operated and complete a cycle in under 60 seconds. Each airlock can hold ten Duran 500ml bottles.

A bespoke trolley with storage is included with the Whitley A155 HEPA.

Whitley A155 HEPA Workstation Features:

• Two removable fronts enable the transfer of larger batches of samples or pieces of equipment for use inside the chamber.
• Patented oval ports enable you to work comfortably, gloved or bare-handed.
• Each port includes an isolator switch so that you can use one port at a time if required. The fact that there are two sets of ports means that two researchers can work in the chamber side-by-side.
• Colour, touch-screen control panel for ease of use and for visual display of parameters such as temperature, humidity, and airlock cycle status.
• Two new options are available: a rotating turntable (up to 3 per shelf) to hold up to 80 x 90mm Petri dishes; and a movable platform to facilitate the movement of heavy items of equipment inside the chamber.
• Other options include a double internal power socket, front loading single sample entry system, cable glands, shelves with optional storage trays, Anaerobic Conditions Monitor and data logging.
• A fully comprehensive maintenance and breakdown contract is also available to prolong the life of your investment. (Note: different arrangements apply in different countries).

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Whitley A135 GMP Anaerobic Workstation

Don Whitley Scientific has over 40 years of experience in the design and manufacture of anaerobic cabinets, and in 2013 developed the Whitley Internal HEPA Filtration System, primarily to serve our research customers. In collaboration with customers running GMP licensed facilities, we have now developed innovative uni-directional airflow technology, and successfully added this feature to an anaerobic workstation to create a unique product offering: a uni-directional airflow workstation that allows users to process samples under strict anaerobic conditions.

The A135 GMP Anaerobic Workstation is specifically designed to be used as a clean-air isolator in processes following Good Manufacturing Practice (GMP). This microbiology isolator provides Grade A air cleanliness (EU GMP), combining sterile uni-directional airflow, positive operating pressure, and physical isolation to provide highly effective product protection. Unlike traditional isolators that use inlet air from the surrounding room, this workstation is a fully closed recirculating system so can be housed in a Grade D cleanroom during GMP compliant use.

Two sizes of airlock are available for this workstation: one is 17.5 litres and provides for the effective transfer of up to 10 x 500ml Duran bottles (or items of a similar size) to and from the chamber in just 2 minutes. The dimensions of this airlock are 197mm x 388mm x 190mm (W x D x H). The larger airlock is 43 litres (measuring 335 x 300 x 300mm) providing effective transfer for items of equipment or for up to 20 x 500ml Duran bottles in just 5 minutes.

With internal working area dimensions of (w x d x h): 1075 x 750 x 608mm, there is a generous amount of working space inside the workstation chamber to house equipment such as centrifuges and homogenisers. The patented oval glove ports provide maximum comfort when used over extended periods.

A135 GMP Anaerobic Workstation Features:

• This Grade A anaerobic isolator maintains strict anaerobic conditions, operating from two gas supplies for the most effective and efficient running costs.
• HEPA filter face velocity/uniformity of uni-directional airflow and Dispersed Oil Particulate (DOP) tests are carried out in accordance with ISO14644-3 to validate system integrity and performance. All tests are performed by an independent organisation.
• The GMP Workstation exceeds the requirements of Grade A air cleanliness (EU GMP) and ISO 14644-1 Class 3 both at rest and in operation.
• Whitley Intelligent Monitoring continually measures atmospheric downflow to ensure optimised filter performance.
• The ‘Standby’ feature (ECO mode) automatically decreases fan speed when the chamber is at rest to maximise energy efficiency.
• The large workstation chamber is perfect for accommodating a variety of items of equipment (removable front fitted as standard).
• A password-protected full-colour touch screen interface allows easy monitoring of all parameters simultaneously. Alarms and status notices are also displayed.
• This workstation is Ethernet-enabled for remote access review and control.
• Data logging system records all monitored parameters every 60 seconds.
• The Event Log lists events showing the date and time they occurred. The log retains information for 100 days and can be downloaded if the Data Logging option has been purchased.
• Air sampling ports can be fitted to allow monitoring systems to be connected to the workstation for continuous particulate monitoring and microbial air sampling.
• A fully automated de-humidification system is included as standard. It does not require any operator intervention.
• A bespoke trolley with heavy-duty castors is provided as standard. A trolley not only saves bench space but is also useful for ease of mobility for service and maintenance.
• Other optional features are available to tailor the system to your requirements.
• The workstation can also be run aerobically if required.
• Range of possible decontamination protocols, including the use of vaporised hydrogen peroxide.

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Whitley H25 Hypoxystation

The Don Whitley Scientific H25 Hypoxystation creates hypoxic and anoxic conditions within a controlled and sustained workstation environment. For cell and tissue culture researchers who want a small chamber with stable conditions in which to accurately control oxygen, carbon dioxide, temperature and humidity, this workstation is ideal.

With the H25 you can control oxygen in 0.1% increments up to 20%; carbon dioxide in 0.1% increments up to 15%; and relative humidity up to 80%. This provides a great deal of flexibility in your research and confidence in your results.

The H25 Hypoxystation can accommodate 145 x 96 well plates while still leaving more than adequate space to process samples. This workstation is equipped with sleeved ports and has a built-in, rapid airlock that can accommodate 3 x 500ml Duran bottles.

If you need to aspirate biological liquid waste from inside the chamber, order with a Vacuum Take-off Port and the Whitley AsPROvac for the perfect package.This workstation is also available with a motorised height adjustable trolley, developed specifically to raise and lower the workstation to suit different users.

H25 Hypoxystation features:

• Built-in rapid airlock allows items to be transferred into the workstation atmosphere in 20 seconds and the doors are interlocked so cannot be opened simultaneously.
• Colour, touchscreen control panel for ease of use and for visual display of parameters such as oxygen level, carbon dioxide level, temperature, humidity, and airlock cycle status.
• Low gas consumption.
• Fully integrated gas control avoids bulky add-on systems and reduces the need for additional bench space.
• Optional 15cm single plate entry system fitted to the side of the workstation.
• Optional data download facility and oxygen profiling.
• Can be fitted with a range of cable glands and other options to tailor the workstation to your requirements.

Download the Whitley Hypoxystation Range Brochure

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Whitley H35 Hypoxystation

The Don Whitley Scientific H35 Hypoxystation creates hypoxic and anoxic conditions within a controlled and sustained workstation environment. It is ideal for cell and tissue culture researchers wanting to accurately control oxygen, carbon dioxide, temperature and humidity. Applications for this instrument include cancer research, neurology and cardiovascular research, as well as many other types of cell culture work.

With the H35 you can control oxygen in 0.1% increments up to 20%; carbon dioxide in 0.1% increments up to 15%; and relative humidity up to 80%. This provides a great deal of flexibility in your research and confidence in your results.

The H35 is also available as an H35 HEPA Hypoxystation, a cell culture chamber ideal for those requiring HEPA filtration, as well as being able to accurately control parameters. Levels of atmospheric cleanliness inside the workstation exceed the requirements of ISO 14644 Class 3. We can provide proof of atmospheric cleanliness.

If you need to aspirate biological liquid waste from inside the chamber, order a Vacuum Take-off Port and the Whitley AsPROvac for the perfect package.

An ideal solution for Seahorse Analyzer users is to purchase the H35 along with the new Whitley i2 Instrument Workstation. These two workstations can be connected via the Whitley Transfer Tunnel, enabling the preparation of cell lines under hypoxic conditions and their transfer directly into the i2 without exposure to air.

H35 Hypoxystation Features:

• This cell culture incubator is available with removable front to facilitate thorough cleaning and the transfer of bulk samples or larger pieces of equipment for use in the workstation.
• Built-in rapid airlock to ensure samples can be transferred into the workstation atmosphere as soon as possible (only takes 60 seconds).
• Colour, touchscreen control panel for ease of use and for visual display of parameters such as oxygen level, carbon dioxide level, temperature, humidity, and airlock cycle status.
• Available with fully automatic humidification system so you can add moisture AND maintain a sterile environment.
• Fully integrated gas control avoids bulky add-on systems and reduces the need for additional bench space.

Download the Whitley Hypoxystation Range Brochure

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Whitley H85 Hypoxystation

The Don Whitley Scientific H85 Hypoxystation creates hypoxic and anoxic conditions within a controlled and sustained workstation environment. It is ideal for researchers wanting to accurately control oxygen, carbon dioxide, temperature and humidity in a variety of cell culture applications.

Now you can control oxygen in 0.1% increments up to 20%; carbon dioxide in 0.1% increments up to 15%; and relative humidity up to 80%, which provides flexibility in your research and confidence in your results.

This workstation is available with an optional refrigeration unit designed to operate at 8°C (other set temperatures available if required). You can also now purchase the H85 with the Whitley Internal HEPA Filtration System.

The H85 is now available as an H85 HEPA Hypoxystation, fitted with the unique Whitley Internal HEPA Filtration System. All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly.

If you need to aspirate biological liquid waste from inside the chamber, order with a Vacuum Take-off Port and the Whitley AsPROvac for the perfect package.

H85 Hypoxystation Features:

• Available with patented oval glove ports operated by a wireless footswitch to allow you to work gloved or bare-handed.
• Built-in 36-litre airlock for transfer of bulk samples and equipment into the workstation environment.
• Colour, touch-screen control panel for ease of use and for visual display of parameters such as oxygen level, carbon dioxide level, temperature, humidity, and airlock cycle status.
• Available with additional options such as HEPA filtration – levels of atmospheric cleanliness inside the workstation exceeds the requirements of ISO 14644 Class 3.
• Fully integrated gas control avoids bulky add-on systems and reduces the need for additional bench space.
• Reminders to perform calibration can be set for between 7 and 21 days.

Download the H85 Hypoxystation Installation Requirements

Download the Whitley Hypoxystation Range Brochure

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Whitley H45 Hypoxystation

The Don Whitley Scientific H45 Hypoxystation provides the facility to mimic in vitro conditions, providing a continuous cell culture environment that eliminates cellular stress linked to variations in temperature, pH levels and oxidation, yielding better cell lines.

You can now control O₂ in 0.1% increments up to 20%; CO₂ in 0.1% increments up to 15%; and relative humidity up to 80%. This provides flexibility in your research and confidence in your results.

This hypoxic chamber has two sleeved ports on a removable front and one instant access port, and has sufficient space to accommodate a variety of equipment inside the chamber, whilst still providing a generous working and incubation area.

The H45 is also available as an H45 HEPA, equipped with the Whitley Internal HEPA Filtration System. All the atmosphere inside the chamber passes through the filter hundreds of times an hour, which ensure the chamber environment is cleaned quickly.

If you need to aspirate biological liquid waste from inside the chamber, order with a Vacuum Take-off Port and the Whitley AsPROvac for the perfect package.

Applications for this instrument include cancer research, IVF, stem cell research, cardiovascular research, as well as many other types of cell and tissue culture work.

An ideal solution for Seahorse Analyzer users is to purchase the H45 along with the new Whitley i2 Instrument Workstation. These two workstations can be connected via the Whitley Transfer Tunnel, enabling preparation of cell lines under hypoxic conditions and their transfer directly into the i2 without exposure to air.

H45 Hypoxystation Features:

• Three portholes provide convenient access to the entire incubation and working areas of the chamber.
• The removable front facilitates thorough cleaning and the transfer of bulk samples or larger pieces of equipment for use in the workstation.
• The built-in rapid airlock ensures samples can be transferred into the workstation atmosphere as soon as possible (only takes 60 seconds).
• Colour, touchscreen control panel for ease of use and for visual display of parameters such as O₂ level, CO₂ level, temperature, humidity and airlock cycle status.
• Ethernet-enabled for remote access to the touchscreen.
• Available with a fully automatic humidification system so you can add moisture AND maintain a sterile environment.
• Fully integrated gas control avoids bulky add-on systems and reduces the need for additional bench space.

Download the Whitley Hypoxystation Range Brochure

Download the Porthole Options Brochure

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Whitley H95 Hypoxystation

The Don Whitley Scientific H95 Hypoxystation has been designed specifically for researchers requiring the ability to accurately control O₂, CO₂, temperature and humidity. It allows the user to control O₂, in 0.1% increments from 0.1% up to 20%; control CO₂, in 0.1% increments from 0.1% to 15%; carry out straightforward calibration of the oxygen sensor; control temperature between 5°C above ambient and 45°C; and to control relative humidity at 80% RH or higher.

The H95 has a chamber capacity of 600 litres and an airlock capacity of 36 litres. The airlock is fitted with an automatic internal door and provides an easy and rapid method for the transfer of up to 133 x 96 well plates or 252 x T25 culture flasks from the laboratory to the workstation. The airlock cycle takes only 5 minutes.

The H95 is now available as an H95 HEPA Hypoxystation, fitted with the unique Whitley Internal HEPA Filtration System. All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly.

If you need to aspirate biological liquid waste from inside the chamber, order with a Vacuum Take-off Port and the Whitley AsPROvac for the perfect package.

H95 Hypoxystation Features:

• Fully integrated gas mixing
• Colour touchscreen interface allows easy monitoring of all parameters simultaneously
• Ethernet enabled for remote access to touchscreen
• Multifunctional porthole system – 2 pairs of ports so can be used by 2 users at the same time
• Each port acts as a mini airlock to transfer small items of lab ware
• Available with automatic sleeve gassing system
• Patented oval gloveports to allow bare handed or gloved operation and maximum comfort in use
• Automated de-humidification as standard
• Available with the Whitley HEPA Filtration System
• A wide range of options and accessories to tailor the system to your particular working conditions.

Download the Whitley Hypoxystation Range Brochure

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Whitley H55 Hypoxystation

The Don Whitley Scientific H55 Hypoxystation, like our other Hypoxystations, provides the facility to mimic in vivo conditions, providing a continuous cell culture environment that eliminates cellular stress linked to variations in temperature, pH levels and oxidation, yielding better cell lines. This workstation has two airlocks and allows you to control O₂ in 0.1% increments up to 20%; CO₂ in 0.1% increments up to 15%; and relative humidity up to 80%. This provides flexibility in your research and confidence in your results.

The H55 has four oval, sleeved portholes and two airlocks (one on the left and one on the right). Two people can easily work side-by-side in this chamber, in the same environmental conditions.

A HEPA filtered version of the H55 Hypoxystation is also available to provide levels of atmospheric cleanliness inside the workstation that exceed the requirements of ISO 14644 Class 3. All the atmosphere inside the chamber passes through the filter hundreds of times an hour, which ensure the chamber environment is cleaned quickly. The Enhanced Biological Containment system can also be specified. This option provides additional safeguards in reducing the possibility of bacteria contained inside workstations being released via the main chamber exhaust valve and the airlock exhaust valve.

If you need to aspirate biological liquid waste from inside the chamber, order with a Vacuum Take-off Port and the Whitley AsPROvac for the perfect package.

Options include:

• Single plate entry system
• Data logging and oxygen profiling capability
• Internal electrical sockets

If you want to be able to operate your workstation with two different temperature levels, specify the dual temperature option. This provides a central divider between the workstation chambers allowing you to select different temperatures for each side. A manually operated sliding door between the two chambers allows items to be passed from one area into the other.

Applications for this instrument include cancer research, stem cell research, cardiovascular research, as well as many other types of cell and tissue culture work.

H55 Hypoxystation Features:

• Has a capacity of 600 litres.
• Removable front as standard on the right-hand half of the unit to facilitate the transfer of bulk samples or larger pieces of equipment for use inside the workstation.
• The two rapid airlocks can each accommodate up to 4 x 96-well plates or 84 x T25s. They enable items to be withdrawn at either end of the workstation offering maximum flexibility and improving the workflow in your laboratory. Both airlocks can be operated at the same time and take only 60 seconds.
• Colour, touchscreen control panel for ease of use and for visual display of parameters such as O₂ level, CO₂ level, temperature, humidity and airlock cycle status.
• Ethernet-enabled for remote access to the touchscreen.
• Comes complete with a custom-designed trolley.
• Fully integrated gas control avoids bulky add-on systems and reduces the need for additional bench space.
• Automatic commissioning cycle to save you time and resources.

Download the Whitley Hypoxystation Range Brochure

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Whitley H135 HEPA Hypoxystation

The Don Whitley Scientific H135 HEPA Hypoxystation is a tall, wide, deep hypoxic chamber. This Hypoxystation has a capacity of 900 litres and can accommodate a variety of items of equipment such as live cell imaging devices, microscopes, plate readers, etc.

The generous internal height facilitates easy pipetting. All cell manipulations can be performed without removing them from your required hypoxic conditions. The entire cell culture incubator chamber is a working and incubation space.

The H135 HEPA Hypoxystation provides precise environmental control whilst leaving plenty of room to work, incubate and conduct analysis. A rapid 16 litre airlock – no risk of compromising conditions inside the chamber (and it’s flushed with nitrogen as an additional cost saving).

If you need to aspirate biological liquid waste from inside the chamber, order with a Vacuum Take-off Port and the Whitley AsPROvac for the perfect package.

For those who want to use a Seahorse XF Analyzer in their work, there is the option of connecting the H135 HEPA to a Whitley i2 Instrument Workstation.

H135 HEPA Hypoxystation Features:

• Precise control of oxygen, carbon dioxide and nitrogen – provides flexibility in your research and confidence in your results.
• The Whitley Internal HEPA Filtration System with optional Enhanced Biological Containment.
• A removable front (fitted with two patented oval ports) to facilitate the transfer of large items of equipment for use inside the chamber.
• Innovative full colour touch screen that is Ethernet-enabled for remote access. Now you can log into your workstation when you are away from the laboratory.
• Fully automated oxygen calibration routine (Patent No. 3109635) – activated via the touchscreen with no need to touch or remove the sensor – saves time, no risk of user error and ensures the accuracy of your results.
• Options Include the Whitley Automatic Humidifier – sterile, on-demand moisture addition.

Download the Whitley Hypoxystation Range Brochure

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Whitley H155 HEPA Hypoxystation

The Don Whitley Scientific H155 HEPA Hypoxystation is the tallest, widest, deepest hypoxic chamber in the Whitley Workstation range. It has a usable internal capacity of 1,900 litres and can accommodate a variety of items of equipment such as live cell imaging devices and microscopes.

The generous internal height facilitates easy pipetting and all cell manipulations can be performed without removing them from your required hypoxic conditions. The H155 HEPA Hypoxystation is equipped with the Whitley Internal HEPA Filtration System. Levels of atmospheric cleanliness inside the workstation exceed the requirements of ISO 14644 Class 3 and we can provide proof of atmospheric cleanliness.

This workstation provides precise environmental control whilst leaving plenty of room to work, incubate and conduct analysis. Two rapid 16 litre airlocks prevent compromising conditions inside the chamber when you are entering and exiting. The airlocks operate independently and take only 60 seconds to run a cycle. Each airlock can accommodate 10 x 500ml Duran bottles.

If you need to aspirate biological liquid waste from inside the chamber, order with a Vacuum Take-off Port and the Whitley AsPROvac for the perfect package.

H155 HEPA Hypoxytation Features:

• Precise control of oxygen, carbon dioxide and nitrogen – provides flexibility in your research and confidence in your results.
• The Whitley Internal HEPA Filtration System with optional Enhanced Biological Containment.
• Two removable fronts (each fitted with two patented oval ports) facilitate the transfer of large items of equipment for use inside the chamber.
• Innovative, full colour touch screen that is Ethernet-enabled for remote access. Now you can log into your workstation when you are away from the laboratory.
• Fully automated oxygen calibration routine (patent pending) – activated via the touchscreen with no need to touch or remove the sensor – saves time, no risk of user error and ensures the accuracy of your results.
• Other options include double internal power socket, refrigeration, front loading single sample entry system, cable glands and storage trays.

Download the Hypoxystation Range Brochure

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Whitley i2 Instrument Workstation

The Whitley i2 Instrument Workstation has been developed in response to a rising number of enquiries from scientists who desire to use Seahorse Extracellular Flux (XF) Analyzers in hypoxic conditions and were dissatisfied with the solutions available. Rather than adapting an existing tissue culture incubator for this purpose, Don Whitley Scientific developed a new tissue culture incubator specifically to suit the precise requirements of Seahorse Bioscience.

Extensive research and careful consideration have gone into the development of the i2, which has resulted in a modular system that includes a number of novel and unique features. This workstation can be used as a stand-alone unit or connected to a Whitley Hypoxystation via the Whitley Transfer Tunnel, enabling the preparation of cell lines under hypoxic conditions and their transfer directly into the i2 without exposure to air.

The Whitley i2 Instrument Workstation is equipped with a generous working area to conduct preparatory work. It is supplied with a removable front, internal mains sockets, and a wireless footswitch to control the patented oval ports.

Another unique feature is the 4-litre integral incubator. This temperature-controlled storage area enables you to precondition cell ware and incubate plates/media at 37°C, under the same atmospheric conditions as the XF Analyzer.

If your research requires unsurpassed accuracy, reproducibility and reliability, then insist on a Whitley Workstation.

Whitley i2 Instrument Workstation Features:

• The i2 maintains an internal temperature no higher than 28°C, excludes carbon dioxide, and provides precise oxygen control.
• The 12 litre airlock, with a cycle time of just 60 seconds, accommodates up to 7 x 0.5 litre bottles, 44 × 96 well plates or 84 × T25 tissue culture flasks plus numerous other flasks, pipettes and laboratory consumables.
• The Whitley i2 Instrument Workstation is one of a range of larger Whitley Workstations developed to allow customers to use a variety of items of specialised equipment under modified atmospheric conditions.
• The combination of a Whitley i2 Instrument Workstation and Seahorse XF Analyzer permits simultaneous, real-time analysis of mitochondrial respiration and glycolysis in mammalian cells under precisely controlled hypoxic conditions.

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Whitley H135 GMP Hypoxystation

The Don Whitley Scientific H135 GMP Hypoxystation is specifically designed to be used as a clean-air isolator in processes following Good Manufacturing Practice (GMP). This workstation provides Grade A air cleanliness (EU GMP) and combines sterile hypoxic laminar airflow, positive operating pressure, and physical isolation to provide highly effective product protection. Due to being a completely closed, re-circulating, isolator system, this workstation can be housed in a Grade D cleanroom during GMP compliant use.

The full-time environmental control offered by the H135 GMP Hypoxystation maximises the reproducibility of the cell-manufacturing processes carried out inside. Further, this hypoxic isolator is designed to be modular and can be combined in different ways to form a production line that ensures that the cell-culture environment is never broken from the beginning to the end of the manufacturing process.

Each workstation is tested to ensure compliance with the relevant portions of ISO 14644-3. In addition, tests carried out in our own laboratories confirm the integral HEPA filtered laminar flow system meets the requirements set out in BS EN 12469:2000 with regard to protection of the working area from particulate and microbial contamination.

Two sizes of airlock are available for this workstation: one is 17.5 litres and provides for the effective transfer of up to 10 x 500ml Duran bottles (or items of a similar size) to and from the chamber in just 60 seconds. The airlock’s internal dimensions are 197mm x 388mm x 190mm (W x D x H). The larger airlock is 43 litres (measuring 335 x 300 x 300mm) providing effective transfer for items of equipment or for up to 20 x 500ml Duran bottles in just 5 minutes.

The patented oval glove ports provide maximum comfort when used over extended periods.

Don Whitley Scientific engineers are trained to carry out filter integrity (DOP) testing on HEPA filters. This allows us to provide full validation on Whitley GMP Hypoxic Workstations (Hypoxystations). Customers who work to GMP processes can rest assured that they don’t have to source their own external, specialist organisations to perform these important tests once their workstation has been installed. DWS has invested in the relevant equipment and training to provide this one-stop solution. Customers can have the confidence that their workstation has been tested by engineers who are fully trained in the operation and manufacture of Whitley Workstations.

With internal working area dimensions of (w x d x h): 1075 x 750 x 608mm, there is a generous amount of working space inside the workstation chamber to house equipment such as large scale bioreactors and imaging systems.

If you need to aspirate biological liquid waste from inside the chamber, order a Vacuum Take-off Port and the Whitley AsPROvac for the perfect package.

H135 GMP Workstations Features:

• Precise oxygen control (between 0.1% and 20.9%) and CO₂ control (between 0.0% and 15.0%).
• Precise control of humidity (up to 80% RH) and temperature (ambient to 40°C).
• H14 HEPA filters remove all particulate providing better than 99.995% efficiency (MMPS).
• The large workstation chamber is perfect for accommodating a variety of items of equipment, which can be installed and removed

Download the Whitley Hypoxystation Range Brochure

Download the Whitley GMP Workstation Brochure: Oxygen Controlled Isolators

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Whitley M35 Workstation

The Don Whitley Scientific M35 Workstation is a 4-gas system with built-in oxygen, carbon dioxide and hydrogen sensors. Precise gas concentrations can be programmed, which is perfect for manipulating samples in a sustainable microaerobic environment. Whitley M Range workstations are extremely flexible in use, allowing anoxic/anaerobic, microaerobic or hypoxic atmospheres to be created, if desired, without the need to change incoming gases.

These workstations were originally designed for the study and isolation of microaerophilic organisms such as Campylobacter spp, Helicobacter pylori and other similarly fastidious organisms. However, due to the ability to control the atmospheric from 0% to 20% oxygen, these systems are widely used as both microbiology and cell culture workstations.

As with our other microaerobic and anaerobic workstations, the Whitley M35 Workstation can be specified with the Whitley Automatic Humidification System. This system is especially beneficial in the cultivation of microaerophilic bacteria such as Campylobacter spp. Our own laboratory tests have indicated that Campylobacter colonies grow larger and exhibit more typical morphology when incubated in an atmosphere with elevated humidity (at least 75% RH).

A range of optional accessories, such as data logging, a single sample entry system, and removable front and cable glands are available to tailor this workstation to your particular requirements.

M35 Workstation Features:

• The M35 can accommodate up to 600 x 90mm Petri dishes.
• A rapid, 12-litre airlock that can transfer 40 plates in only 60 seconds.
• Intuitive colour touchscreen interface with PIN-code-protected user access levels.
• Ethernet-enabled for remote access to the touchscreen.
• Data download facility that allows you to record workstation parameters and download stored data in seconds for traceability and reference.
• NEW – motorised height adjustable trolley is now available for the Whitley M35.

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Whitley H155 GMP Hypoxystation

The Don Whitley Scientific H155 GMP Hypoxystation is specifically designed to be used as a clean-air, hypoxic isolator in processes following Good Manufacturing Practice (GMP). This workstation allows two people to work in the chamber at same time and is specifically designed to accurately control the parameters of O₂, CO₂, temperature, and humidity.

Providing Grade A air cleanliness (EU GMP), this hypoxic isolator combines sterile laminar airflow, positive operating pressure, and physical isolation, to provide highly effective product protection. Due to being a completely closed, re-circulating, isolator system, this workstation can be housed in a Grade D cleanroom during GMP compliant use.

The full-time environmental control offered by the H155 GMP Hypoxystation maximises the reproducibility of the cell-manufacturing processes carried out inside. Further, this Hypoxystation is designed to be modular and can be combined in different ways to form a production line that ensures that the cell-culture environment is never broken from the beginning to the end of the manufacturing process.

Each workstation is tested to ensure compliance with the relevant portions of ISO 14644-3. In addition, tests carried out in our own laboratories confirm the integral HEPA filtered laminar flow system meets the requirements set out in BS EN 12469:2000 with regard to protection of the working area from particulate and microbial contamination.

This workstation is equipped with two integral airlocks (one at either side of the main workstation chamber) providing an ideal layout for a ‘production line’ style of working. Two sizes of airlock are available: 17.5 litres or 43 litres. These airlocks have been specifically designed to aid the transfer of serological pipettes into the workstation. Each 17.5 litre airlock is capable of accommodating 10 x 500ml Duran bottles and the internal dimensions are 197mm x 388mm x 190mm (W x D x H). The 43 litre airlocks (measuring 335 x 300 x 300mm) can each accommodate up to 20 x 500ml Duran bottles.

The patented oval glove ports can be fitted with your choice of gauntlet and provide maximum comfort when used over extended periods.

With internal working area dimensions of (w x d x h): 2200 x 750 x 620mm, there is a huge amount of working space inside the workstation chamber to house equipment such as large scale bioreactors and imaging systems.

H155 GMP Workstation Features:

• Precise control of oxygen between 0.1% and 20.9%.
• CO2 control between 0.0% and 15%; humidity control (up to 80% RH); and temperature control (ambient to 40°C).
• H14 HEPA filters remove all particulate providing better than 99.995% efficiency (MMPS).
• Whitley Intelligent Monitoring continually measures atmospheric down flow to ensure optimised filter performance.
• Two sizes of airlock available: 17.5 litres or 43 litres.
• ‘Standby’ feature automatically decreases fan speed when the chamber is at rest to maximise energy efficiency.
• The large workstation chamber is perfect for accommodating a variety of items of equipment, which can be installed and removed via the removable front (fitted as standard).
•  Ethernet-enabled for remote access review and control.
• Four oval ports and two airlocks allow two people to work in the chamber at the same time.
• Data logging system records all monitored parameters every 60 seconds
• The Event Log lists events showing the date and time they occurred. The log retains information for 100 days and can be downloaded if the Data Logging option has been purchased.
• A fully automated de-humidification system is included as standard. It does not require any operator intervention.
• A bespoke trolley with heavy-duty castors is provided as standard. A trolley not only saves desk space but is also useful for ease of mobility for service and maintenance.
• Range of possible decontamination protocols, including the use of vaporised hydrogen peroxide.
• Air sampling ports can be fitted to allow particulate monitoring systems to be connected to the workstation for continuous particulate monitoring.
• Other optional features are available to tailor the system to your requirements.

Download the Whitley GMP Workstation Brochure: Oxygen Controlled Isolators

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Whitley M45 Workstation

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Whitley M85 Workstation

The Don Whitley Scientific M85 Workstation variable atmosphere workstation is a 4-gas system with built-in oxygen, carbon dioxide and hydrogen sensors. This is an extremely flexible system as it allows users to programme precise gas concentrations and use the workstation with anoxic/anaerobic, microaerobic or hypoxic atmospheres without the need to change incoming gases.

Whitley M Range Workstations were originally designed for the study and isolation of microaerophilic organisms such as Campylobacter spp, Helicobacter pylori and other similarly fastidious organisms. Due to the flexibility of the atmospheric control (from 0% to 20% oxygen), these systems are now widely used for both microbiology and cell culture applications.

The large 36 litre airlock with powered internal door provides effective sample and equipment transfer in the fastest possible time whilst ensuring the minimum amount of oxygen is introduced into the chamber. Two oval ports enable you to work gloved or bare handed.

The M85 Workstation can also be specified with the Whitley HEPA Filtration System.

A range of optional accessories, such as data logging, motorised adjustable height trolley, and cable glands are available to tailor this workstation to your particular requirements.

M85 Workstation Features:

• Each porthole also acts as a mini-airlock so you can transfer up to 10 plates each time you insert or withdraw your arms, so reducing airlock cycles and gas usage.
• Chamber accommodates between 540 – 610 x 90mm Petri dishes.
• Colour, touch-screen control panel for ease of use and for visual display of parameters such as temperature, humidity, and airlock cycle status.
• Optional refrigeration unit available if you are working with organisms that need low temperature incubation or storage conditions.
• Reminder to perform calibration can be set for between 7 and 21 days.
• Automatic commissioning cycle to save you time and resources.

Download the Anaerobic and Microaerobic Workstations Brochure

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Whitley M55 Workstation

The Don Whitley Scientific M55 Workstation is a 4-gas system that provides a huge working capacity when there is a need to process, culture and examine samples in a sustainable, variable atmosphere environment. It has built-in oxygen, carbon dioxide and hydrogen sensors. This allows users to programme precise gas concentrations, which is perfect for manipulating samples in a sustainable environment. The Whitley M Range of workstations provide the most flexibility of any models in the Don Whitley Scientific range; allowing anoxic/anaerobic, microaerobic or hypoxic atmospheres to be created if desired without the need to change incoming gases.

M Range Workstations were originally designed for the study and isolation of microaerophilic organisms such as Campylobacter spp, Helicobacter pylori and other similarly fastidious organisms. Due to the flexibility of the atmospheric control (from 0% to 20% oxygen), these systems are now widely used for both microbiology and cell culture applications.

The M55 Workstation has two airlocks (one on the left and one on the right) and has a huge capacity of 600 litres. Two people can easily work side-by-side in this chamber, in the same environmental conditions.

If you want to be able to operate your workstation with two different temperature levels, specify the dual temperature option. This provides a central divider between the workstation chambers allowing you to select different temperatures for each side. A manually operated sliding door between the two chambers allows samples to be passed from one area into the other.

A range of optional accessories, such as HEPA Filtration, cable glands, power sockets or a single sample entry system, are available to tailor this workstation to your requirements.

M55 Workstation Features:

• Two rapid, 12-litre airlocks can transfer 40 plates each in only 60 seconds.
• Intuitive colour touchscreen interface with PIN-code-protected user access levels.
• Data download facility that allows you to record workstation parameters and download stored data in seconds for traceability and reference.
• Accommodates up to 1,200 x 90mm Petri dishes.
• Removable front as standard on the right-hand half of the unit to facilitate the transfer of bulk samples or larger pieces of equipment for use inside the workstation.
• Colour, touchscreen control panel for ease of use and for visual display of parameters such as temperature, humidity, and airlock cycle status.
• Ethernet-enabled for remote access to the touchscreen.
• Comes complete with a custom-designed trolley.
• Optional conventional, oval, sleeved ports or a mixture of oval and instant access ports.
• Automatic commissioning cycle to save you time and resources.

Download the Anaerobic and Microaerobic Workstations Range Brochure

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Whitley M95 Workstation

The Don Whitley Scientific M95 Workstation is a 4-gas system with built-in oxygen, carbon dioxide and hydrogen sensors. Precise gas concentrations can be programmed, which is perfect for manipulating samples in a sustainable microaerobic environment. Whitley M Range workstations are extremely flexible in use, allowing anoxic/anaerobic, microaerobic or hypoxic atmospheres to be created, if desired, without the need to change incoming gases.

This workstation is ideal for the study and isolation of Campylobacter spp, Helicobacter pylori and other similarly fastidious microaerophiles, however, due to the flexibility of the atmospheric control (from 0% to 20% oxygen), these systems are also widely used for both microbiology and cell culture applications.

Whitley Microaerobic Workstations provide the most flexibility of any models in the Don Whitley Scientific range, allowing anoxic/anaerobic, microaerobic or hypoxic atmospheres to be created if desired without the need to change incoming gasses.

The large 36 litre airlock with powered internal door provides effective sample and equipment transfer in the fastest possible time whilst ensuring the minimum amount of oxygen is introduced into the chamber.

The M95 Workstation is now available as an M95 HEPA, fitted with the Whitley Internal HEPA Filtration System. All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly, providing levels of atmospheric cleanliness inside the workstation that exceed the requirements of ISO 14644 Class 3.

M95 Workstation Features:

• Huge chamber capacity of up to 1,400 x 90mm Petri dishes.
• Four oval ports enable you to work gloved or bare-handed.
• Each porthole also acts as a mini-airlock so you can transfer up to 10 plates each time you insert or withdraw your arms, so reducing airlock cycles and gas usage.
• Colour, touch-screen control panel for ease of use and for visual display of parameters such as temperature, humidity, and airlock cycle status.
• A reminder to perform calibration can be set for between 7 and 21 days.
• Optional unique HEPA Filtration System.
• Available with three different types of single plate entry systems.
• Automatic commissioning cycle to save you time and resources.

Download the Anaerobic and Microaerobic Workstations Brochure

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Whitley M135 HEPA Workstation

The Don Whitley Scientific M135 HEPA Workstation is a tall, wide, deep microaerobic chamber. It is a 4-gas system with built-in oxygen, carbon dioxide and hydrogen sensors. This allows users to programme precise gas concentrations, which is perfect for manipulating samples in a sustainable variable atmosphere environment. The Whitley M Range of workstations allows anoxic/anaerobic, microaerobic or hypoxic atmospheres to be created, if desired, without the need to change incoming gases.

Whitley M Range Workstations were originally designed for the study and isolation of microaerophilic organisms such as Campylobacter spp, Helicobacter pylori and other similarly fastidious organisms. Due to the flexibility of atmospheric control (from 0% to 20% oxygen), these systems are now widely used as both microbiology and cell culture workstations. As with our other microaerobic and anaerobic workstations, the Whitley M35 can be specified with the Whitley Automatic Humidification System. This system is especially beneficial in the cultivation of microaerophilic bacteria such as Campylobacter spp. Our own laboratory tests have indicated that Campylobacter colonies grow larger and exhibit more typical morphology when incubated in an atmosphere with elevated humidity (at least 75% RH).

The M135 HEPA Workstation has an internal volume of 900 litres and can accommodate a variety of items of equipment such as imaging devices, microscopes, plate readers, spiral platers, etc. It comes complete with the unique Whitley Internal HEPA Filtration System. All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly.

The generous internal height facilitates easy pipetting. All your work can be performed without removing your samples from the set environmental conditions and the entire chamber can be used as both working and incubation space.

With a rapid 16-litre airlock, there is no risk of compromising conditions inside the chamber (and it’s flushed with nitrogen as an additional cost saving).

DWS also offers two optional cooling methods for this workstation. A Refrigeration System could be useful if you are working with organisms that need low-temperature incubation. Alternatively, the Heat Removal System allows the workstation to maintain ambient temperatures at all times.

A range of optional accessories, such as data logging and cable glands are available to tailor this workstation to your particular requirements.

H135 HEPA Workstation Features:

• Two patented oval ports enable you to work comfortably, gloved or bare-handed.
• Rather than use off-the-shelf HEPA filters, DWS has worked with one of the world’s leading filter manufacturers to develop a filter specifically tailored to suit this workstation.
• Levels of atmospheric cleanliness inside the workstation exceed the requirements of ISO 14644 Class 3 (equivalent to Class 1 of the US Fed Std 209E Cleanroom Standards and exceed the particle counts attained at rest’ (without an operator) of Grade A as set out in the GMP Guidelines). We can provide test results confirming particulate levels inside the workstation.
• Fitted with a fan that circulates the internal atmosphere continuously – contributing to even temperature distribution and ensuring any internal particulate will be in motion.
• A removable front enables the transfer of larger batches of samples or pieces of equipment for use inside the chamber.
• Colour, touch screen control panel for ease of use and visual display of parameters such as temperature, humidity and airlock cycle status.
• Other options to tailor the workstation to your requirements include a single sample entry system; cable glands; shelves and data logging.

Download the Anaerobic and Microaerobic Workstations Brochure

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Whitley Jar Gassing System

With the Whitley Jar Gassing System you can create perfect conditions for growing anaerobes in jars in just 2 minutes for less than 18 pence and for microaerophiles in 15 seconds for less than 3 pence (compared with about £1.60 for every gas generating kit used).

A full colour touch screen control panel allows you to monitor, in real time, that the criteria necessary for the creation of either anaerobic or microaerobic conditions has been met.

The Whitley Jar Gassing System has an built-in fault detector to create an alert if a jar is leaking. It is essential that jars remain air tight to maintain conditions and that you have confidence that the jar will maintain the correct conditions throughout the incubation period. As the Jar Gassing System pulls a vacuum, it detects the jar is sealed by the speed that the vacuum is pulled (if the jar is leaking it will not be able to create a vacuum).

If you already have anaerobic jars that you wish to use you can order the adaptor kit, which will enable you to connect this anaerobic jar system to most types of jar.

Whitley Jar Gassing System Features:

• Reduces the cost of creating microaerobic conditions by 98% and anaerobic conditions by 89%.
• Reduces the time taken to create required conditions – gas packs can take up to an hour but the Jar Gassing system removes the atmosphere and replaces it with anaerobic mixed gas very quickly.
• It is easy to use (with instructions in English, German and French) so no complicated operator training is required.
• Jar uses low-temperature, re-usable catalyst sachets.
• Incorporates PIN code-protected user access levels for additional security.
• The optional printer provides you with a hard copy audit trail for accreditation purposes.

Download Anaerobic Range Brochure

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Anaerobic Jars

For simple cultures of anaerobic and microaerophilic organisms, we have both stainless steel jars and the transparent, stackable Whitley Incubation Box, which are all robust and easy to use. The traditional stainless steel jars have a positively located lid fitted with the unique Whitley Schrader® Valve System and carrier clips for Low-Temperature Catalyst Sachets.

Types of Anaerobic Jars:

Stainless Steel Jars for use with the Whitley Jar Gassing System:

• a 9 Plate Jar with a 3-litre capacity  (Order Code: A05075)
• a 48 Plate Jar with a 10-litre capacity (Order Code: A05076)

Stainless Steel Jars for use with gas packs:

• a 9 Plate Jar with a 3-litre capacity  (Order Code: A00005)
• a 48 Plate Jar with a 10-litre capacity (Order Code: A00002)

And the transparent, stackable Incubation Box (Order Code: A09740)

All our anaerobic jars are ideal for use with the Whitley Jar Gassing System.

The Whitley Jar Glassing System can create perfect conditions for growing anaerobes in jars in just 2 minutes for less than 18 cents and for microaerophiles in 15 seconds for less than 3 cents. It also has a built-in fault detector to create an alert if a jar is leaking. It is essential that jars remain airtight to maintain conditions and that you have confidence that the jar will maintain the correct conditions throughout the incubation period. As the Jar Gassing System pulls a vacuum, it detects the jar is sealed by the speed that the vacuum is pulled (if the jar is leaking it will not be able to create a vacuum).

Download the Whitley Anaerobic Jar Leaflet

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Whitley Incubation Box

New from Don Whitley Scientific Incubation Box is the rectangular, transparent and stackable anaerobic ‘jar’. It can be used to create the perfect environmental conditions for the isolation and cultivation of anaerobic and microaerobic organisms and for cell culture applications.

The Whitley Incubation Box is ideal for use with the Whitley Jar Gassing System (evacuation/replacement method) or as a stand-alone system to use with gas generation sachets.

Because of the shape of the vessel, and as no connectors or valves protrude beyond the outer surface of the lid, Whitley Incubation Box’s can be stacked on top of each other inside an incubator or on the bench, saving valuable space. The main jar (without the lid in place and with no samples inside) can be nested with other empty jars so that work in progress takes up minimal space.

This anaerobic ‘jar’ will accommodate either 12-14 x 90mm Petri dishes or 5 x standard multi-well plates. The four latches used to open and close the box are attached to the lid so there are no loose parts to misplace.

The Whitley Incubation Box may be used to transfer items into and out of a Whitley Workstation and has been designed to fit through the 35-sized airlock and every larger Whitley airlock. This application is ideal when you want to move samples from one workstation to another and when you want to preserve your samples in modified atmospheric conditions while they continue to grow in an external incubator.

The design of the Whitley Incubation Box includes a safety valve, which ensures an appropriate level of vacuum is achieved without damaging the box itself. It is durable and lightweight and can be used to create multiple atmospheres.

Whitley Incubation Box Features:

• Accommodates both Petri dishes and multi-well plates.
• Both lids and bases can be nested for storage.
• Flexibility to create different atmospheric conditions.
• Latches are permanently attached to the lid so there are no loose parts to misplace.
• The completed box can be stacked on top of each other for incubation.
• The rectangular shape saves space in the incubator.
• Includes a safety valve to ensure an appropriate level of vacuum is achieved without damaging the box itself.
• Durable and lightweight.

Download Anaerobic Range Brochure

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Whitley WASP Touch®

The Don Whitley Scientific WASP Touch ® is a precision laboratory instrument designed to deposit accurate volumes of liquid sample in an Archimedes spiral onto the surface of a rotating agar plate. Spiral plating is well documented and validated as an effective method for the enumeration of microorganisms.

Designed for the needs of modern microbiology laboratories, the WASP Touch® is a fundamentally different spiral plater with no need for a separate vacuum source. With touchscreen operation the system is extremely simple to use and provides real cost savings and process improvements.

Whether you are working in the field of dental research, food microbiology, calculating antimicrobial concentration (MICs) or studying the microbiome, this robust, reliable device could really save time and money in your laboratory.

WASP Touch® provides Automated Intelligent Monitoring Software (AIMS) to guide you through set-up and daily check routines – ensures consistent trouble-free plating. Precise quantities of sanitising fluids are dispensed automatically to maintain optimum levels. This ensures effective and efficient sanitisation takes place.

The deposition profiles possible with WASP Touch® allow bacterial and fungal colonies to be enumerated over a 100,000-fold (5 log₁₀) range, from a minimum of 20 cfu/ml in 1000 μl pour plate mode to a maximum of 2 x 10⁶ cfu/ml in 10 μl logarithmic mode. An optional Extended Volume Package is available, however, to allow for a greater number of replicates plates, linear deposition profile and a wider range of deposition profiles.

Spiral plating techniques are recognised in ISO 4833-2 & 7218 as well as the AOAC 977.27 standard.

Optional fully comprehensive maintenance and breakdown contracts are available to prolong the life of your investment. (Note: different arrangements apply in different countries).

WASP Touch ® Features:

• Patented automatic sanitising system automatically maintains correct liquid levels in sanitising solutions.
• Illuminated, covered working area.
• Easy serviceability.
• The sample may be aspirated from beakers, bottles and tubes – providing maximum flexibility in use.
• Automatically log date, time, deposition, user name, and number of plates produced – a powerful diagnostic tool.
• Flowsense – detects the movement of liquid through the WASP Touch®.
• Sanitising system reservoirs are available in two sizes and can contain sufficient fluid for hundreds of wash cycles – perfect for busy laboratories.
• Fully integrated vacuum waste system – occupies the smallest amount of bench width.
• Side connectors for ease of access.

Download the WASP Touch ® Brochure

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Vacuum Source 602

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Whitley AsPROvac

The Whitley AsPROvac is an all-in-one laboratory aspirator to ensure biological liquid waste can be aspirated and disposed of quickly and safely. Whether it is the delicate removal of supernatants or the rapid emptying of larger culture flasks, this flexible system has 15 vacuum levels to alter the aspiration speed. A range of aspiration tools is provided to enable you to aspirate from a variety of vessels.

AsPROvac features a novel, quick-release cap/lid assembly, a shatterproof 4-litre autoclavable waste bottle and a digital display of the vacuum level.

The hand-held aspirator comprises a reusable single tip and an adaptor for single-use tips along with a reusable 8-channel tip and an 8-channel adaptor for single-use tips.

The AsPROvac laboratory vacuum system is ideal for use with a Whitley Hypoxystation (that has a vacuum take-off port fitted).

The AsPROvac laboratory vacuum system has the following features:

… to provide a method of safely aspirating and disposing of biological liquid waste:

• Self-closure connectors avoid the escape of liquids and aerosols.
• Equipped with a novel, quick-click lid – with no multi-turn screw that the previous user has over-tightened!
• Supplied with a 4-litre, shatterproof waste bottle.
• Liquid level ‘full’ indicator.
• Features a hydrophobic filter for user protection.
• All parts that come into contact with the liquid waste are autoclavable.
• Fitted with a handle to make it easy to carry the waste bottle and empty the contents.
• A reliable, high-quality, all-in-one system with no need for any additional components.

… to provide ease of use for laboratory staff …

• Simple, straightforward operation with little training required.
• Colour-coded, quick-release tubing connectors so you always assemble the product correctly and quickly.
• The integral display shows the vacuum level selected – perfect for speeding up the emptying of large vessels or reducing the level for the delicate and careful removal of smaller quantities of biological liquid waste.
• A compact footprint with the bottle set low in the holder to provide a solid, stable base and to prevent bottles from being knocked over.
• Lightweight so can be moved around the laboratory with ease.
• Additional ergonomic hand holds on the base for easy lifting and handling of the entire unit.

Download the AsPROvac Leaflet

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